水稻矮缩病毒的检测和介体传毒能力初步分析

Research on the Delection of Rice Dwaif Virus

快速从水稻叶片和单头介体昆虫中检测水稻矮缩病毒的斑点分子杂交。与10%SDS-PAGE检测方法相比,不仅敏感、快速、简单,可以检测田间批量样品,用于病害流行研究和测报,而且可以用于介体叶蝉传毒能力的分析。研究表明:用本地黑尾叶蝉分别接种RDV本地分离物和云南分离物,斑点杂交显示介体带毒率相似,分别为84%、75%,但生物学接种结果差异相当大,分别为28.1%、3.8%,另外斑点杂交显示云南病区的黑尾叶蝉带毒率为88%,说明介体叶蝉的传毒能力具有地域性,介体叶蝉带毒率与传毒能力也存在一定差异。

For the detection of Rice dwarf virus（RDV ） from single leafhopper and leaf, the dot-blotted hybridization method based on molecular biology were established. Compared with 10% SDS- PAGE detection,dot- blotted hybridization was fast,simple and sensitive,and could be applied for the study on epidemiology and prediction of diseases caused by RDV and also for the study on the transmitting ability of leafhopper. The results showed： after local leafhoppers were inoculated with RDV- SX isolate, RDV- LQ isolate, the vector viruliferous rates were similar as 84 % and 75% respectively,but the rice incidence rates were dissimilar as 28.1% and 2.8 % respectively. Otherwise the vectors from Yunnan endemic sites were 90 % viruliferous. These indicated the transmission ability of vector leafhopper was regional and varied with the vector viruliferous rates.