摘要
目的:观察同型半胱氨酸(Hcy)对大鼠脑微血管内皮细胞(RCMEC)损伤的保护作用.方法:将RCMEC分为损伤组和正常对照组,观察Hcy对RCMEC的影响;另将RCMEC分为正常对照组、损伤组和丙丁酚不同剂量组,观察各组细胞乳酸脱氢酶(LDH)释放率及细胞生长曲线.结果:与正常对照组比较,损伤组RCMEC生长受抑且细胞LDH释放率明显增加;丙丁酚浓度在10～50μmol/L范围内细胞LDH释放率依次减少.结论:Hcy对RCMEC有毒性损伤作用,丙丁酚呈剂量依赖性拮抗Hcy所致RCMEC损伤.
OBJECTIVE： To observe the protective effect of homocysteine（Hcy） on rats＇ cerebral microvascular endothelial cells （RCMEC） injury. METHODS： RCMEC were divided into injury group and normal control group, the effect of Hey on which was observed;Besides, RCMEC were divided into normal control group, injury group and probucol groups of different dosage, the lactate dehydrogenase （LDH） release rate and the cells growth curve in each group were observed. RESULTS： As compared with the normal control group, the growth of RCMEC in the injury group was inhibited and the release rate of LDH increased remarkably ;The release rate of LDH decreased in turn when probucol was in a concentration range of 10-50μmol/L. CONCLUSION： Hey can cause toxic injury to RCMEC, probucol assumes dose dependent counteraction on Hey- induced injury.
出处
《中国药房》
CAS
CSCD
北大核心
2005年第24期1857-1858,共2页
China Pharmacy
基金
广西壮族自治区卫生厅资助课题(Z2002098)
