PTEN在子宫内膜样腺癌组织中的表达及意义

The expression and significance of PTEN in endometrioid adenocarcinoma

目的:检测第10染色体同源丢失性磷酸酶张力蛋白基因(phosphatase and tension homology delet echromo-someten,PTEN)在不同类型子宫内膜腺上皮细胞中的表达。方法:采用免疫组化检测不同类型子宫内膜组织中PTEN的表达。结果:(1)PTEN的表达在增生期和分泌期子宫内膜无差异(P>0.05)。(2)不典型增生与单纯性增生、不典型增生与复杂性增生相比,PTEN表达均有显著性差异(P<0.01),而单纯性增生与复杂性增生之间无显著差异(P>0.05)。(3)子宫内膜样腺癌中:尽管PTEN表达在<50岁年龄组大于≥50岁组,≤1/2肌层浸润深度组大于>1/2组,但其间均无显著性差异(皆P>0.05)。随着癌细胞分化程度的降低和分期的升高,PTEN表达有逐渐降低的趋势,但不同分化程度的癌组织之间和Ⅰ期、Ⅱ期与Ⅲ～Ⅳ期之间均无显著差异(P>0.05)。结论:PTEN表达降低促使了癌前病变的发生,可能是引起内膜细胞癌变的机制之一,PTEN表达与临床病理学特征关系不大。

Objective： To analyze the expression of （phosphatase and tension homology deleted chromosometen, PTEN） in the gland epithelial cells of various endometriums. Methods： Using immunohistochemistry technique to detect PTEN expression in different kinds of endometriums. Results： （1）The differences were not significant for the PTEN expression in proliferative endometrium （PE） and secretory endometrium （SE）（P〉0.05）. （2）The differences were significant between atypical hyperplasia （AH） and simple hyperplasia （SH） ,and also between AH and complex hyperplasia （CH） （P〉0.01）. （3）Though PTEN expression were more in [〈50] groups than [≥50] groups in the endometrioid adenocarcinoma （EAC）, and also more in [≤ 1/2] groups of depth of myometrial invasion （DMI） than [〉1/2] groups the differences are not significant. With the differentiation becoming poor and the clinical stages elevating, PTEN expression were decreasing,those are： Ⅰ 〉 Ⅱ 〉Ⅲ -Ⅳ;well differential endometrioid adenocarcinoma （WDEA）〉 moderate differential endometrioid adenocarcinoma （MDEA）〉 low differential endometrioid adenocarcinoma （LDEA）, but the differences are not significant（P〉0.05）. Conclusion： Loss of PTEN expression may put important effect in progression of EH and may be involved in the development of EAC. PTEN are not correlated with histopathological characters of EAC. KEY WORDS Endometrioid adenocarcinoma;PTEN; Immunohistochemistry