SUR1基因多态性与2型糖尿病的相关性研究

Correlation between polymorphism of SUR1 gene and type 2 diabetes

目的:探讨2型糖尿病(T2DM)与磺脲类药物受体1(SUR1)基因多态性的关系。方法:随机提取正常人136例(正常组)及2型DM患者173例(T2DM组)外周血白细胞基因组DNA,PCR扩增SUR1基因24号内含子多态性位点的区域,用限制性酶切片段长度多态性分析其基因型,并用测序证实不同酶切图形基因型的准确性。结果:T2DM组和正常组SUR1基因第24号内含子基因型及等位基因分布差别有统计学意义(P<0.05)。结论:SUR1基因可能是中国天津地区2型糖尿病发生的易感基因。

Objective： To study the relationship between polymorphism and human sulfonylurea receptorl （SUR1） gene in type 2 diabetes mellitus （T2DM）. Methods： 136 normal subjects and 173 patients with T2DM in Tianjin were included. Genomic DNA was extracted from white cells of peripheral blood. The aim fragment containing polymorphism site was amplified by PCR and then was digested by restrictive enzymes （PCR-RFLP）.The mutations were sequenced to verify the consistency. Results： The mutation frequency in intron 24 in normal subjects was significantly higher than that in patients with T2DM（P〈0.05）. Conclusion： SUR1 gene is possibly a susceptible gene for T2DM in Tianjin.