HBV相关性肝细胞癌肝组织蛋白质组差异的初步分析

Preliminary study on differential proteomic analysis of hepatic tissues in HBV related hepatocellular carcinoma

目的分析 HBV 相关性肝细胞癌蛋白质组与癌周组织(肝硬化组织、慢性肝炎组织)蛋白质组之间的差异.鉴定其中的差异蛋白质点。方法应用双向聚丙烯酰胺凝胶电泳.对18例肝细胞癌组织与12例肝硬化组织、6例慢性肝炎组织的蛋白质组表达谱进行差异分析,应用基质辅助激光解吸离子化飞行时间质谱仪对差异蛋白质点进行了鉴定。结果肝细胞癌组织与癌周组织的对比中.共发现有表达水平存在显著差异的47个蛋白质点.17个差异蛋白质点得到了初步鉴定,其中8个蛋白质点在既往文献中未曾报道其与肝细胞癌变相关。结论慢性肝炎基础上发生的癌变与肝硬化基础上发生的癌变,其癌变机制存在差别。本实验研究中初步鉴定的差异蛋白质,可能有助于HBV 相关性肝细胞癌的癌变机制、肿瘤标记和治疗靶标的进一步研究。

Objective To analyze proteome difference of hepatic tissues obtained from HBV related hepatocellular carcinoma（HCC） ： tumorous and surrounding-tumorous tissue（including cirrhotic and chronic hepatitis tissue） ,and to identify the differential protein spots. Methods Differential proteome profiles of 18 tumorous tissues, 12 cirrhotic tissues, and 6 chronic hepatitis tissues were analyzed using two-dimensional polyacrylamide gel electrophoresis（2-DE）, differential protein spots were submit to further identify using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF-MS）. Results Comparing the tumorous tissue with surrounding-tumorous tissue, a significant change in expression level was found in 47 protein spots, among which 17 proteins were identified preliminarily. Eight proteins have not been reported to associate with HBV related HCC in the literature previously. Conclusions The hepatocarcinogenesis of HBV related HCC developed on cirrhosis is different with which developed on chronic hepatitis. The proteins revealed in this experiment may be useful in the future for studies pertaining to the mechanisms of hepatocarcinogenesis, or as diagnostic markers and therapeutic targets for HBV related HCC.