青蒿琥酯诱导人白血病细胞K562凋亡的实验研究

The apoptosis-inducing effect of artesunate on human leukemia K562 cells

目的观察青蒿琥酯对人白血病细胞株K562抑制增殖和凋亡作用。方法通过MTT显色法观察不同浓度青蒿琥酯对K562的生长抑制作用,通过AnnexinV/PI双标记法检测青蒿琥酯对K562细胞的凋亡诱导作用。结果青蒿琥酯在1～8μgml浓度范围内能分别明显抑制K562细胞的增殖,并具有时间和浓度的依赖性;在2～8μgml浓度范围内青蒿琥酯诱导K562细胞凋亡具有明显的量效关系;4μgml浓度组的青蒿琥酯在0～72h内,诱导K562凋亡具有明显的时效关系。结论青蒿琥酯在体外能抑制K562细胞的增殖,可能是通过诱导其发生凋亡而起作用。

Objective To investigate the proliferation inhibition and apoptosis-inducing effect of Artesunate on human leukemia K562 cells. Methods MTT assay was undertaken to observe the growth inhibition of K562 ceils exposed to Artesunate. The apoptosis of K562 ceils was detected by cell morphological observation, DNA agarose gel electmphoresis,Annexin V/PI double labeling and intracellular DNA content analysis. Results Artesunate markedly inhibited the proliferation of K562 cells in a time-dependent and dose-dependent manner at the range of 1 to 8 μg/ml.Flow cytometry showed that artesunate induced apoptosis of K562 cells in a time- and dose- dependent manner within 0-72 hours （4 μg/ml）, and within the concentration rar/ge of 2 to 8 μg/ml （at 48 hours）. Conclusion Artesunate could inhibit the proliferation and induce apoptosis of human leukemia K562 cells in vitro.