摘要
目的研究广东人群CYP2C9基因第四外显子的多态性。方法PCR扩增50例健康广东人之CYP2C9基因跨越第四外显子的DNA片断,测序后截取完整的第四外显子序列进行blast分析。结果检测的50个广东人的CYP2C9基因第四外显子序列均与GenBank中进入号为AY702706.1的相应序列完全一致。结论广东人群CYP2C9基因第四外显子的基因序列高度保守,无多态性。
Objective To investigate the exon 4 polymorphism of the CYP2C9 gene in Guangdong population. Methods Using the DNA of 50 healthy individuals in Guangdong population as the templates , the DNA fragment containing the complete exon 4 of CYP2C9 gene was amplified by polymerase chain reaction, the PCR products were subjected to direct sequencing and the sequence of exon 4 was drawn to analyze with blast procedure . Results The exon 4 sequences of CYP2C9 gene of the 50 individuals were identical to the published sequence in GenBank (accession number: AY702706.1 ). Conclusion The exon 4 of CYP2C9 gene is highly conserved in Guangdong population and shows no polymorphism.
出处
《广东药学院学报》
CAS
2005年第6期712-714,共3页
Academic Journal of Guangdong College of Pharmacy
基金
广东省自然科学基金(034051)
广东省医学科研基金(A2003347)
广东药学院科研基金(2002272)