温度、pH和摄食作用对西施舌胃蛋白酶活力的影响

The effects of temperature,pH and feeding time on pepsin activity of Coelomactra antiquate

研究了不同温度、不同pH值和摄食后不同时间条件下西施舌胃蛋白酶的活性变化。结果表明:在pH为3.0时,西施舌胃蛋白酶最适温度为50℃,明显高于生理温度,酶活力达到最大(290.18±20.11)U;在温度为50℃下,最适pH值为2.6,酶活力达到最高,为(426.59±30.80)U,远低于生理pH;西施舌在摄食后,胃蛋白酶的活力有明显的提高,摄食后0.5 h西施舌胃蛋白酶的活力达到最大值(96.04±30.02)U,其后活力降低,但在摄食后4 h时,活力又有回升,之后又趋于最低值(43.06±6.69)U。

In the paper, the pepsin activity of Coelomactra antiquate was studied at different temperatures, pH and different time after feeding. The results indicate that： optimum temperature for C. antiquate pepsin is 50 ℃ in fixed pH 3.0,and pepsin activity is highest and amounts to （290.18 ± 20.11） U. At fixed temperature 50 ℃, the most suitable pH is 2.6, and pepsin activity value reachs （426.59 ±30.80） U. After feeding, the pepsin activity increases and at 0.5 h reaches highest value （96.04 ± 30.02） U, then the activity declins. But after feeding 4 h, the activity rises again, then declines to lowest value （43.06 ± 6.69） U.