HPLC测定8位肝癌病人5-FU血药浓度及其药物动力学研究

Determination of 8 Hepatic Cancer′s 5-FU Concentration in Serum by HPLC,and its Study Pharmakinetics

目的用改进的HPLC法测定8位肝癌病人静滴5-FU后7个时间点的血药浓度,对其进行药物动力学研究。方法采用HPLC-紫外检测5-FU血药浓度;并用3P97药动学程序计算出一系列药物动力学参数。结果血药浓度个体差异极大(2.5倍以上);5-FU药时曲线符合开放式二房室模型,AUC与年龄成正比。结论5-FU静脉滴注给药,血药浓度个体差异较大,临床效果(疗效、毒性)的差异也很大,故必须实施TDM(治疗药物监测),制订个体化给药方案,使用药更安全、有效、合理和经济,副作用更小。

Objective To determine the concentration,and study the Phamacokinetics of 5-FU in serum at seven time points from 8 Hepatic Cancer＇s by HPLC. Methods HPLC-UV was applied to determine the concentration of 5-FU in serum. A series of parameters were analysed by 3P97 Pharmacokinetics procedure. Result The concentration-time curve of 5-FU was fit to a TWO- compartment model. AUC was directly proportional to age. Individual difference was significant （above 2.5 multiple）. Conclusions The individual difference of serum concentration was rather significant as well as clinical effect after 5-FU in administration. The TDM should be used for the individual administration, which will make the drug administration. To be more safe, effective, reasonable, economical and side effect less.