十二烷基硫酸钠对人牙周膜细胞活性影响的实验研究

An experimental study of effect of SDS on the activity of human PDLC

目的:观察不同浓度十二烷基硫酸钠(sod ium dodecyl su lfate,SDS)对人牙周膜细胞(perio-dontal ligam ent cell,PDLC)活性的影响,探讨牙膏中存在的去垢成分SDS是否对人体健康存在着潜在危害。方法:采用细胞培养技术及四唑盐(MTT)比色方法,检测加入SDS后与对照组比较细胞活性的变化。结果:PDLC在加入100μg/mL SDS后,细胞活性与正常组比较已明显降低,在SDS浓度≥200μg/mL时PDLC已全部死亡。SDS浓度≤25μg/mL对PDLC生长无明显影响。结论:SDS在低浓度时即对PDLC有明显的细胞毒作用。

AIM ：To observe the effect of sodium dodecyl sulfate （SDS） on the activity of human periodontal ligament cells （PDLC） ill different concentrations. METHODS：The cell euhure technique and MTT method were used to observe the effect of SDS on tile activity of PDLC. RESULTS：Comparing with the control group,the activity of PDLC has greatly decreased after adding 100 μg/mL SDS, When the concentrations of SDS add up to 200 μg/mL,all PDLC died, But when SDS＇s concentrations are less than 25μg/mL it has no inhibiturv effect on PDLC＇s activity, CONCLUSION： These results indicated that even in lower concentrations of SDS ean inhibit the activity of PDLC in vitro.