摘要
目的:初步探讨新基因m cpr1在小鼠胚胎多组织发育中的表达模式。方法:取妊娠(gestationday,GD)12、14、18 d的C57BL/6N孕鼠,拉颈处死,取出胎鼠,利用已制备的MCPR1多克隆抗体,进行免疫组织化学染色,分析MCPR1的表达情况。结果:免疫组化结果显示:MCPR1在小鼠胚胎多组织的发育中呈现不同的表达模式,在小鼠胚胎14 d(E14)时,M eckel软骨内有少数MCPR1阳性表达细胞,软骨周围阳性细胞较多;在E18时,M eckel软骨内MCPR1阳性表达细胞增多,且前部较后部多。在胚眼发育不同的时期也有不同的表达分布。结论:推测m cpr1基因在颅神经嵴细胞成骨过程中,可能以促PCD基因的身份在软骨细胞的移行中发挥作用;胚眼发育过程中m cpr1基因可能在视泡及晶状体等眼部结构的发生发展中起着一定的作用。
AIM:Toinvestigate the expression of mouse cleft palate related gene in different patterns during murine embryonic development.METHODS:Gestation day(GD)12,14,18 C57B1/6N pregnant female murine were sacrificed.The fetus were immtediately removed from uterus and were detected in polyclonal antibodies of MCPR1 by immunohistochistry to investigate the expression of MCPR1.RESULTS:The results of immunohistochemistry showed that bthe MCPR1 expressed extedsively in different patterns during murine embryonic development,however there was no tissue specification or obvious tendency.CONCLUSION:MCPR1 expressed differently in different tissue with different patterns.According to which it could be inferred that the MCPR1 might take part in the development of many organs during different periods of embryonic development.
出处
《牙体牙髓牙周病学杂志》
CAS
2005年第12期660-662,共3页
Chinese Journal of Conservative Dentistry
关键词
腭裂相关基因
免疫组化
胚胎发育
cleft palate related gene
immunohistochemistry
embryonic development