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MCPR1在小鼠胚胎发育中的表达 被引量：1

Expression of MPCR1 during murine embryonic development
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摘要 目的:初步探讨新基因m cpr1在小鼠胚胎多组织发育中的表达模式。方法:取妊娠(gestationday,GD)12、14、18 d的C57BL/6N孕鼠,拉颈处死,取出胎鼠,利用已制备的MCPR1多克隆抗体,进行免疫组织化学染色,分析MCPR1的表达情况。结果:免疫组化结果显示:MCPR1在小鼠胚胎多组织的发育中呈现不同的表达模式,在小鼠胚胎14 d(E14)时,M eckel软骨内有少数MCPR1阳性表达细胞,软骨周围阳性细胞较多;在E18时,M eckel软骨内MCPR1阳性表达细胞增多,且前部较后部多。在胚眼发育不同的时期也有不同的表达分布。结论:推测m cpr1基因在颅神经嵴细胞成骨过程中,可能以促PCD基因的身份在软骨细胞的移行中发挥作用;胚眼发育过程中m cpr1基因可能在视泡及晶状体等眼部结构的发生发展中起着一定的作用。 AIM：Toinvestigate the expression of mouse cleft palate related gene in different patterns during murine embryonic development.METHODS：Gestation day（GD）12,14,18 C57B1/6N pregnant female murine were sacrificed.The fetus were immtediately removed from uterus and were detected in polyclonal antibodies of MCPR1 by immunohistochistry to investigate the expression of MCPR1.RESULTS：The results of immunohistochemistry showed that bthe MCPR1 expressed extedsively in different patterns during murine embryonic development,however there was no tissue specification or obvious tendency.CONCLUSION：MCPR1 expressed differently in different tissue with different patterns.According to which it could be inferred that the MCPR1 might take part in the development of many organs during different periods of embryonic development.
作者 丰培勋 轩东英 金岩 顾晓明 雷战军
机构地区 武警陕西总队医院 第四军医大学口腔医学院 武警总医院
出处 《牙体牙髓牙周病学杂志》 CAS 2005年第12期660-662,共3页 Chinese Journal of Conservative Dentistry
关键词 腭裂相关基因 免疫组化 胚胎发育 cleft palate related gene immunohistochemistry embryonic development
分类号 Q786 [生物学—分子生物学]
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参考文献5

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  • 1[1]Li X,Jin Y,Dong SZ,Yue W,Li Y.Effects of the proliferation in palatal mesenchymal cells during the development of cleft palate.Zhonghua Kouqiang Yixue Zazhi(Chin J Stomatol) 2001; 36(6):430
  • 2[2]Murray J. Gene/environment causes of cleft lip and/or palate.Clin Genet 2002;61(4):248－ 56
  • 3[3]Yue H, Yu JP. Effect of regulatory factor of cell cycle p27kip1 and cyclinE proteins on the genesis and progression of human pancreatic cancer.Zhongguo Linchuang Kangfu（ Chin J Clin Rehabil） 2002; 6(20):3124－ 5
  • 4[4]Tao HR, Wang QP, Zhang YY, Liu JZ, Li J. Clong and expression of ODF/OPGL/RANKL gene related to osseous absorption disease.Zhongguo Linchuang Kangfu（ Chin J Clin Rehabil） 2002; 6(20):3128－ 9
  • 5[5]Abbott BD, Schmid J, Brown JG, Wood CR, White RD, Buckalew AR, Held GA.RT PCR Quantification of AHR, ARNT,GR, and CYP1A1 mRNA in Craniofacial Tissues of Embryonic Mice Exposed to 2,3,7,8 Tetrachlorodibenzo p dioxin and Hydrocortisone.Toxicological Sciences 1999;47:62－ 75
  • 6[6]Ueno M, Nakayama H, Kajikawa S, Katayama K, Suzuki K, Doi K.Expression of ribosomal protein L4 (rpL4) during neurogenesis and 5 azacytidine(5AzC) induced apoptotic process in the rat.Histol Histopathol 2002; 17(3):789－ 98
  • 7[7]Nishida J,Shiratsuchi A,Nadano D,Sato TA,Nakanishi Y. Structural change of ribosomes during apoptosis: degradation and externalization of ribosomal proteins in doxorubicin treated Jurkat cells.J Biochem(Tokyo) 2002;131(3):485－ 93
  • 8[8]Rossman TG,Wang Z.Expression cloning for arsenite resistance resulted in isolation of tumor suppressor fau cDNA: possible involvement of the ubiquitin system in arsenic carcinogenesis.Carcinogenesis 1999; 20:311－ 6
  • 9[9]Sahali D,Pawlak A,Valanciute A,et al.A novel approach to investigation of the pathogenesis of active minimal change nephrotic syndrome using subtracted cDNA library screening.J Am Soc Nephrol 2002;13(5):1238－ 47
  • 10Zhongliang Z, Xin H, Nan L, et al. Direct cloning of cell differential expression genes with full-length by a new strategy based on the multiple rounds of long distance polymerase chain reaction beads mediated subtraction[J]. J Biotechnology, 1999,73: 35-

共引文献5

同被引文献7

  • 1轩东英,金岩,金明,王浈,王亦菁,胡世颉.腭裂相关基因mcpr1在不同组织细胞中的表达[J].牙体牙髓牙周病学杂志,2004,14(10):553-555. 被引量：1
  • 2轩东英,金岩,金明,轩昆,邢向辉,郑梁,赵征.新基因mcpr1在小鼠牙胚发育中的时空表达和意义[J].实用口腔医学杂志,2005,21(6):730-732. 被引量：1
  • 3李鑫,金岩,刘源,王新文.MCPR-1基因在小鼠腭突组织及各脏器中的表达及意义[J].现代口腔医学杂志,2006,20(1):35-37. 被引量：1
  • 4Dong - Ying Xuan, Xin Li, Zhi - Hong Deng, et al. Identification and characterization of a novel gene, Mcprl and its possible function in cell proliferation of embryonic palatal mesenchymal ceils[ J ]. Journal of Biological Chemistry, 2006,281 ( 45 ) : 33997 - 34008.
  • 5TuckerAS, Sharpe PT. Molecular genetics of tooth morphogenesis and patterning: the right shape in the right place[ J]. J Dent Res,1999, 78(4) : 826 - 834.
  • 6NeubuserA, Peter H, Bailing R, et al. Antagonistic interactions between FGF and BMP signaling pathways : a mechanism for positioning the sites of tooth formation [ J]. Cell, 1997, 90 (2) : 247 - 255.
  • 7Zamore PD, Tuschl T, Sharp PA, et al. RNAi : double - stranded RNA directs the ATP - dependent cleavage of Mma at 21 - 23 nucleotide intervals[ J]. Cell, 2000, 101 ( 1 ) :25 -33.

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牙体牙髓牙周病学杂志
2005年 第12期

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