IBA处理在苹果组培嫩梢生根中的作用

EFFECTION OF IBA ON INDUCICNG ROOTING OF APPLE TISSUE CULTURE

用不同浓度的ＩＢＡ对４个苹果品种的组织培养分化嫩梢进行了不同时间的浸泡处理。结果表明，以ＩＢＡ使用浓度为１００ｍｇ／ｌ处理１２ｈ，置于１／２ＭＳ＋０．１ｍｇ／ｌＧＡ＋１．５ｍｇ／ｌＩＡＡ＋０．５ｇ／ｌ活性碳培养基诱导生根的技术方案最优。红乔纳金等４个苹果品种的平均生根率均达７１．０％以上，最高为９０．８％。

The technology method on inducing apple tissues culture roots was obtained by IBA treatment to seedlings-rootless of 4 apple cultivars,then these seedlings-rootless were put the culture medium of 1/2MS+0.1mg/lGA+1.5mg/lIAA and induced roots.Rooting rates of 4 apple cultivars were above 71.0%,and the highest was 90.8%.