大鼠肝肾联合移植模型术式的改进

A MODIFIED TECHNIQUE OF ESTABLISHING RAT MODEL OF SIMULTANEOUS LIVERo-KIDNEY TRANSPLANTAION

目的探讨大鼠肝肾联合移植动物模型的术式改进方法。方法以0-4℃乳酸钠林格注射液经腹主动脉对供肝和供肾进行原位灌洗。整块切取供鼠肝、肠、胰、脾及与右肾,保存液中修整。移植时血管重建全部采用袖套式吻合,应用支架行胆总管吻合法重建胆道连续性,输尿管带膀胱瓣吻合,建立SD-SD大鼠原位肝肾联合移植模型。结果共行大鼠肝肾联合移植90次,其中预实验50次。正式实验成功率达90%。模型建立降低了难度,具有血管吻合时间短、血流通畅、无肝期短等优点。结论此模型是较为简易、可靠的大鼠肝肾联合移植模型。

Objective To explore a better method of establishing rat model of simultaneous liver-kidney transplantation. Methods In the donor operation, the liver and the right kidney were flushed with 0-4 C sodium lactate Ringer＇s injection through aorta, and en bloc resection of organs of right kidney, pancreas, intestines, liver and spleen were underwent . Preparation was performed in the preservation solution. During the recipient operation, all the blood vessels were reconstructed with cuff methods. The bile duct was anastomosed using a simple inside bracket. Operation of connecting ureter attached part of bladder with the bladder was conducted. Result 90 simultaneous liver-kidney transplant operations including 50 preliminary experiments were carried out and the success rate of formal transplantations was 90%. The time for blood vessel anastomosis and anhepatic stage was reduced. Complications such as stenosis were avoided. Conclusion This rat model of simultaneous liver-kidney transplantation is simple and reliable.