摘要
目的筛选急性髓系白血病细胞多药耐药相关基因。方法在建立HL-60/DNR多药耐药(MDR)细胞系的基础上,采用抑制消减杂交的方法结合基因芯片技术建立HL-60/DNR抑制消减杂交文库,并挑选其中部分克隆进行序列测定和同源性分析。结果HL-60/DNR对多种化疗药物均产生了不同程度的耐受。在所构建的HL-60/DNR抑制消减杂交文库中,经过基因芯片筛选出明显差异表达克隆共有14个;进一步分析发现这些基因在白血病细胞多药耐药中的作用大多未见报道。结论多种基因(已知的或未知的)均参与了急性髓系白血病细胞多药耐药的调节,大规模筛选与克隆这些基因为进一步研究急性髓系白血病细胞多药耐药产生的机制奠定了必要的理论基础。
Objective Try to screen and separate multidrug resistance （MDR） related genes in acute myeloid leukemia cell. Methods After establishing the MDR leukemia cell llne （ HL-60/DNR）, a suppression subtractive hybridization library was established by suppression subtractive hybridization and gene chip. The resulting subtracted eDNA clones were partially sequenced and analyzed by blasm. Results The HL-60/DNR produced MDR to a variety of chemotherapeutic agents. 14 genes were found to express 5-fold difference of expressionlevels between HL-60/DNR and untreated HL-60 cells and were selected to sequence. Most of the 14 sequenced genes have not been reported to be related to MDR in leukemia. Conclusion Many genes both known and unknown were found to relate to the MDR in AML. Discovery of these gene provided a solid foundation to elucidate the mechanism of MDR in acute myeloid leukemia cell.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2005年第6期659-661,665,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
中国博士后科学基金资助项目(No.2004036440)
关键词
白血病
急性髓性
多药耐药
基因克隆
抑制消减杂交
基因芯片
acute myeloid leukemia
multi-drug resistance
gene cloning
suppression subtractive hybridization
gene chip
