洛土辛对失血性休克大鼠的保护作用

Protective Effect of Lotusine on Rats with Hemorrhagic Shock

目的研究洛土辛(Lotusine,Lot)对失血性休克大鼠的保护作用。方法建立大鼠失血性休克模型,进行复苏与药物治疗。采用生理记录仪监测血流动力学,生物化学法测定丙二醛(MDA)、乳酸脱氢酶(LDH)和血气分析。结果Lot(2.5、5、10mg/kg,单次缓慢静脉推注)呈剂量依赖性提升失血性休克大鼠的血压及休克24h的存活率。Lot[5mg/kg首剂缓慢静脉推注,维持量10μg/(kg.min)]和米力农[Mil,0.75mg/kg首剂缓慢静脉推注,维持量3μg/(kg.min)]可显著提高失血性休克大鼠的存活率,其中Lot治疗24h和48h,失血休克大鼠的存活率由对照组25.00%和16.67%提高至83.33%和75.00%。Lot还显著地提高平均动脉压(MAP),改善pH、PaO2、PaCO2、H2CO3和剩余碱(BE)等血气指标,明显降低血浆MDA和LDH。结论洛土辛可改善失血性休克大鼠的心功能障碍,增加心输出量,减轻失血性休克大鼠的细胞损伤,提高大鼠的存活率,对失血性休克大鼠有较好的治疗作用。

Objective To evaluate the protective effects of Lotusine （Lot）, an inhibitor of phosphodiesterase Ⅲ, on the rats with hemorrhagic shock. Methods Mean arterial pressure （MAP） and heart rate （HR） were monitored and arterial blood gases and plasma malondialdehyde （MDA）, lactate dehydrogenase （LDH） were measured at different time points. The survival was observed in 24 and 48 h in the models of hemorrhagic shock rats. Results Lot [ （5 mg/kg, i. v and 10ug/ （kg ·min） drip for the maintenance] could obviously increase survival rate from 25.00 % and 16. 67 % （NS） at 24 h and 48 h after shock to 83.33% and 75.00%~ respectively. Moreover, Lot enhanced MAP and improved arterial blood gases including pH, PaO2, PaCO2, H2CO3 and BE （mmol/L） in hemorrhagic shock rats. Meanwhile, it also significantly reduced plasma MDA and LDH of the shock rats. Conclusion Lot can increase cardiac output, improve metabolic dysfunction and alleviate cellular injury. Therefore, it can enhance survival rate of the rats with hemorrhagic shock.