大鼠肺发育过程中黏着斑激酶表达变化

Changes of Expression of FAK during the Development of Lungs in Rats

目的探讨大鼠肺发育不同阶段黏着斑激酶(FAK)表达的变化。方法取胚胎18、20和21d及出生后1、4、7、10和21d大鼠肺组织标本,采用免疫组织化学法和Western blot技术对FAK蛋白表达进行定位、定量检测,采用RT-PCR方法对FAK mRNA表达水平进行半定量分析。结果在肺发育不同时期,从蛋白、基因水平均证实有FAK表达,以生后第4天表达最强。结论FAK在肺发育过程中发挥重要作用,它可能与气道上皮发育、呼吸管腔和毛细血管网形成密切相关,并参与肺泡上皮细胞增殖、分化。

Objective To explore the changes of expression of focal adhesion kinase （FAK） in different stages of the development of lungs. Methods All lung tissues of fetal and neonatal rats were collected at the following ages： gestational ages （GA） of 18, 20 and 21 days, and 1 ,4, 7, 1 and 21 days after birth. The expression of FAK protein was located and quantitated by immunohistochemistry and Western blot. The expression of FAK mRNA was detected by semi-quantitative reverse transcription polymerase chain reaction （RT-PCR）. Results At the different stages of lung development, the expression of FAK was detectable at protein and gene levels. The expression was strongest on the postnatal day 4. Conclusion FAK may play an important role in the development of lung. It not only modulates the process of development of airway surface epithelium and the morhogenesis of respiratory tube and capillary vessel, but also may be involved in alveolar epithelial proliferation and differentiation.