热休克蛋白90在系统性红斑狼疮外周血单个核细胞中的表达

Expression of HSP90 Protein and mRNA in Peripheral Blood Mononuclear Cells of Patients with Systemic Lupus Erythematosus

目的探讨热休克蛋白90(HSP90)在系统性红斑狼疮(SLE)发病中的作用。方法使用免疫组化SP法和细胞原位杂交技术检测45例SLE患者外周血单个核细胞(PBMC)中HSP90蛋白、HSP90mRNA的表达情况,另取15例健康血样进行正常对照。结果45例SLE患者PBMC中HSP90蛋白阳性表达17例(37.78%),15例正常对照组中未见明显阳性细胞。半定量分析SLE活动组PBMC中HSP90蛋白表达的平均吸光度(A)值为(0.412±0.230),显著高于正常对照组(0.117±0.033)和SLE非活动组(0.187±0.019)(均P<0.01);SLE非活动组也显著高于正常对照组(P<0.05)。45例SLE患者中HSP90mRNA阳性表达20例(44.44%),15例正常对照组中未见阳性细胞。半定量分析SLE活动组PBMC中HSP90mRNA表达的平均A值为(0.350±0.225),显著高于正常对照组(0.114±0.009,P<0.01)和SLE非活动组(0.221±0.040,P<0.05);SLE非活动组与正常对照组比较也显著升高(P<0.05)。SLE中抗心磷脂抗体阳性患者HSP90水平明显高于阴性患者。结论SLE患者中存在HSP90的过度表达,且在抗心磷脂抗体阳性的患者中尤其明显,并与疾病的活动性相关。提示HSP90可能在SLE的发病机制中起着重要作用。

Objective To study the pathogenesis of HSP90 in systemic lupus erythematosus （SLE）. Methods By using immunohistochemistry and in situ hybridization technique, the expression of HSP90 protein and mRNA was detected respectively in peripheral blood mononuclear cells （PBMC） from 45 SLE patients and 15 healthy controls. Results Out of 45 SLE patients, the expression of HSP90 protein in PBMC was detectable in 17 cases （37.78%）. No expression of HSP90 protein was detectable in healthy control group. Semi-quantitative measurement of HSP90 protein expression in the blood specimen revealed that the mean luminosity in active SLE patients, inactive patients and healthy controls was （0. 412±0. 230）, （0. 187±0. 019） and （0. 117±0. 033） respectively. Active SLE patients had higher levels of HSP90 protein expression in PBMC than healthy controls and inactive SLE patients （P〈0.01）, and inactive SLE patients also had higher levels of HSP90 protein expression than healthy controls （P〈0.05）. The expression of HSP90 mRNA in PBMC was detected in 20 cases of 45 SLE patients （44.44%）. No expression of HSP90 mRNA was detected in healthy control group. Semi-quantitative measurement of HSP90 mRNA expression in the blood specimen showed that the mean luminosity in active SLE patients, inactive patients and healthy controls was （0. 350±0. 225）, （0. 221±0. 040） and （0, 114±0, 009） respectively. Active SLE patients had higher levels of HSP90 mRNA expression in PBMC than healthy controls （P〈0.01） and inactive SLE patients （P〈0.05）, and inactive SLE patients also had higher levels of HSP90 mRNA expression than in healthy controls （P〈0.05）. The HSP90 protein level in patients with positive anticardiolipin antibodies was higher that that in negative patients. Conclusion HSP90 in SLE patients is up-regulated, and the protein was notably elevated in patients with positive antieardiolipin antibodies. Moreover, the overexpression of HSP90 is associated with the disease activity. Hence HSP90 is likely to play a crucial role in the pathogenesis of SLE.