CO_2激光对水稻的诱变效应

StudyonmutageniceffectsofriceseedirradiatedbyCO2laser:LuoRongting.

用不同功率密度和时间的ＣＯ２激光辐照处理浙糯２号干、湿两种不同生理状态的种子。从Ｌ１代后期的主要农艺性状分析和Ｌ２的突变率考查表明，激光处理对Ｌ１代的株高、有效穗数、每穗后粒数、千粒重和单株粒重有一定刺激作用，Ｌ２代变异效应明显，除未发现叶绿素突变外，早熟、高秆、矮秆和不育等都有发生。总突变率为０．５１－１．３０％，其突变频率比ｙ射线处理低，但其诱发的早熟突变频率较高，湿种子处理的突变颁率高于干种子。ＣＯ２激光对水稻诱变处理的适宜剂量是：功率密度为１０－１５Ｗ，湿、干种子分别处理３和４秒。

Zhenuo2riceseedswithtwodifferentphysiologicalstateswereirradiatedbyCO2laserindif-ferentpower-densityandradiationtimerespectivcly.ThestudyonmainagronomiccharaclersofL1generationandthemutationfrequencyofL2showedthatplantheight.effectivespikeperplant,grainNo.perpanicle.1000-grainweightandgrainweightperplantofL1generationwerestimulatedbyCO2lasertreatment.Therewerenoanycholorophyllmutationbutearlymaturity.long-stalk.dwarfandstcrilitymutationwereobservedinL2generation.TotalmutalionfrequencyofL2generationwerefrom0.51to1.30percent.andmutationfrequencyofwetseedsirradiatedbyCO2laserwerehigherthanthatofdryseeds.Accordingtotheseresults,wethinkthatitisthebettermutagenicdoseofCO2lasserforustoirradiatericeseedswithpower-densityfrom10-15W/cm2andradiationtimefrom3to4seconds.