高氟低碘对子代大鼠学习记忆及脑乙酰胆碱酯酶的影响

Effects of High Fluoride and Low Iodine on Learning-Memory and TchE of Brain in Offspring Rats

目的研究高氟与低碘对子代大鼠学习记忆行为的影响及其可能的机制。方法断乳Wistar大鼠(雌∶雄=3∶1)32只,随机分为4组:对照组,饲喂大鼠标准饲料,去离子水;高氟组,饲喂大鼠标准饲料,饮100mg/L氟化钠(NaF)去离子水;低碘组,饲喂低碘饲料(定做),去离子水;高氟低碘组,饲喂低碘饲料,饮100 mg/L氟化钠(NaF)去离子水。饲喂3个月。观察子代大鼠在30、60、90 d时学习记忆能力的改变。同期处死,测定脑组织TchE的活性。结果与对照组相比,各实验组子代大鼠在5 min内学习错误次数(EN1)和24 h后的5 min内错误次数(EN2)均有增加(P<0.05)。被动回避的潜伏期时间均有减少(P<0.05)。高氟低碘组子代大鼠比高氟组、低碘组的EN1、EN2均显著增加,潜伏期(ST)有不同程度的减少。高氟低碘组子代大鼠大脑TchE活性在30、60、90 d龄极显著下降(P<0.01)。结论高氟与低碘影响了大脑TchE活性,可能是使学习记忆能力下降的机制之一。

Objective To study the effect of high level fluoride and low level iodine on learning-memory in offspring rats and possible mechanism. Methods Thirty - two Wistar rats were randomly divided into four groups each of eight （female： male = 3 ： 1 ）. The rats were treated with high fluoride （103 and 150 mg NaF/L）, low iodine （0.0855 mg/kg）, or broth high fluoride （103 and 150 nag NaF/L） and low iodine （0.0855 mg/kg） respectively. Results Compared with control rats, error number （EN1） and （EN2） of the experimental offspring rats increased significantly （P 〈 0.05）. Sustaining time （ST） reduced obviously （P 〈 0.05）. EN1 and EN2 of the experimental rats in the group of high fluoride and low iodine were the highest in all groups （ P 〈 0.05）. Acetylcholinesterase （TchE） activity in the group of high fluoride and low iodine decrased significantly at 30, 60 and 90 days （P 〈 0.01 ）. Conclusion The interfered TchE activity of brain by irlgh fluoride and low iodine may be an important mecharism to affect learning-memory ability.