肺癌组织中p53V和p16基因的表达及临床意义

Expression regularity and its clinical significance of gene p53V and p16 in tumor tissue of patients with lung cancer

目的:探讨肺癌组织中p53V和p16基因编码蛋白的表达及其临床意义。方法:采用流式细胞术对47例非小细胞肺癌患者瘤组织p53V+和p16+细胞表达率进行检测和分析,并对肿瘤转移者和未转移者的p53V和p16基因编码蛋白表达水平进行对比研究。结果:肺癌患者瘤组织p53V+细胞检出百分率:肿瘤患者为58.52±26.02,显著高于正常对照的9.55±2.97,t=486.78,P=0.0045,肿瘤转移者为77.87±14.33,显著高于未转移者48.87±23.87,t=521.36,P=0.0071,并随着患者临床分期和病理分级的增加而逐渐增高;肺癌患者瘤组织p16+细胞检出率的变化规律与p53V+的变化恰好相反。两者相关分析显示,p53V+细胞与p16+细胞表达率呈显著负相关,r=-0.648,P=0.0180。结论:肺癌患者瘤组织细胞p53V和p16基因编码蛋白表达水平变化与肺癌临床生物学行为的关系十分密切。p53V和p16的检测可以作为肺癌诊断的参考指标,并可提示肺癌的转移情况,为患者的预后估计提供客观依据。

OBJECTIVE： To study the expression and its clinical significance of tumor related genes p53 and P16 coding protein expression in tumor tissue of patients with lung cancer. METHODS： Expression rates of p53V^＋ and p16^＋ cells were measured and analyzed in tumor tissues of 47 patients with non-small cell lung cancer by flow cytometry. The comparison of expression levels of p53V^＋ and p16^＋ coding protein were studied in tumor tissues between metastasis and non-metastasis patients with lung cancer. RESULTS： The expression rate of p53V^＋ cells in tumor tissues （58.52±26.02） was higher than that of normal control group （9.55 ± 2.97）, t = 486. 78, P = 0. 004 5. The expression rate of p53V^＋ cells of patients with metastasis （77. 87±14. 33） was higher than that of patients with non-metastasis （48. 87± 23.87） ,t=521.36, P=0. 007 1. The expression rate of p53V^＋ cells of the patients increased with the increasement of clinical stages and pathology grades of patients with lung cancer. While the expression regularity of p16^＋ cells in tumor tissues was contrary compared with that of p53V. The correlation analysis showed that the expression rate of p53V＋ cells was negatively correlated to that of p16＋ cells, r = - 0. 648, P = 0. 018 0. CONSLUSIONS： The expression level changes of p53V and p16 genes coding protein in tumor tissues are so closely related to the clinical biological behavior of patients with lung cancer that the measurement of pS3V and p16 genes coding protein may not only be regarded as a reference marker of lung cancer diagnosis, but also provide important information of tumor metastasis and prognosis estimation.