活血化瘀类中药制剂对海马缺血损伤大鼠学习记忆的影响

Effect of Chinese herbs of blood-activating and stasis-dissipating on learning and memory ability of rats with hippocampal ischemia injury

目的:探讨活血化瘀类中药制剂芪丹通脉片对海马梗死模型大鼠学习记忆能力的保护作用。方法:①实验于2004-09/12在第四军医大学西京医院理疗科实验室完成。选用SD雄性健康成年清洁级大鼠48只。随机将大鼠分为6组:空白对照组,假手术组,模型组,芪丹通脉片高、中、低剂量组,每组8只。②除空白对照组和假手术组,其余各组大鼠均采用光化学法诱导大鼠双侧海马梗死模型。假手术组尾静脉注射等体积生理盐水后光纤插入照射。芪丹通脉片(主要成分为黄芪、丹参、桂枝、红花等)高、中、低剂量组分别于术后当日以3.24,1.08,0.36mg/kg的剂量,10mL/kg溶液灌胃7d,1次/d。模型组、假手术组用生理盐水替代等体积灌胃,余同中药治疗组。空白对照组正常饲养。③分别在大鼠术前,术后3,5,7d用Morris水迷宫评价缺血大鼠学习记忆能力,并观察大鼠脑组织病理结构改变。④计量结果差异比较采用单因素方差分析和重复测量数据的方差分析,LSD检验。结果:大鼠48只均进入结果分析。①芪丹通脉各剂量组大鼠脑组织病理结构损伤较模型组轻。②平均逃避潜伏期:术前各组大鼠差异不明显;术后3d,模型组及芪丹通脉各剂量组明显长于空白对照组和假手术组(P<0.05),证明造模成功,芪丹通脉各剂量组明显短于模型组(P<0.05)。术后5和7d,空白对照组和假手术组及芪丹通脉片各剂量组明显短于缺血组(P<0.05)。结论:芪丹通脉片能改善海马缺血大鼠学习记忆能力,对局部脑梗死有保护作用。

AIM： To study the protective effect of qidantongmai on learning and memory ability of rats with bilateral hippocampal infartion. METHODS： ①The experiment was conducted in the Laboratory of Department of Physiotherapy and Rehabilitation, Xijing Hospital of the Fouth Military Medical University. Forty-eight healthy male SD rats were randomized into 6 groups： Blank control group, sham-operated group, model group, high dose of qidantongmai group, middle dose of qidantongmai group and low dose of qidantongmai group with 8 rats in each group. ②All rats except those in the control group and sham-operated group were made into bilateral hippocampal infarction models with photochemistry. Rats in the sham-operated group were radiated models with optical fiber after injected with normal saline of the same volume through caudal vein. Rats in the high/middle/low dose of qidantongmai group received gastric perfusion of 10 mL/kg solution at a dose of 3.24, 1.08 and 0.36 mg/kg respectively, once a day for 7 days. Rats in the model group and sham-operated group received gastric perfusion of normal saline of the same volum, and rats in the blank control group were bred normally.③ Learning and memory ability of rats were assayed with Morris maze before operation and 3, 5, 7 days after operation, and pathological changes of brain tissue were observed. ④ Differences of measurement were compared with single factor analysis of variance as well as repetitive measurements analysis of variance. RESULTS： A total of 48 rats were involved in the analysis of resuhs.① Pathological injury of brain tissue in rats of high/middle/low dose of qidantongmai group were slighter than that in the model group. ②Average escaping latency： There were no significant differences among groups before operation; It was significantly longer 3 days after operation in the model group and all of qidantongmai groups than that in the blank control group and sham-operated group （P 〈 0.05）, which meant the modeling succeeded, and it was longer in the model group than that in all of qidantongmai groups （P 〈 0.05）. At the 5^th day and 7^th day of operation, it was obviously longer in the ischemia group than that in other 5 groups （P 〈 0.05 ）. CONCLUSION： Qidantongmai can ameliorate learning and memory ability of rats with hippocampal ischemia injury , and has a protective effect on local cerebral infartion.