精神分裂症患者5-羟色胺2A受体基因T102C基因型频率的地区差异(英文)

Regional differences of genotype frequency of 5-hydroxytryptamine 2A receptor gene T102C of schizophrenics patients

背景:由于分子遗传学技术的发展,关于精神疾病与分子遗传的研究日趋增多,但结论不尽一致,对5-羟色胺2A受体基因T102C基因型频率分布是否有地区、种族差异尚需要更多证实。目的:分析精神分裂症患者5-羟色胺2A受体基因T102C基因型频率在地理上的分布。设计:以精神分裂症患者为观察对象的抽样调查。单位:常州和平医院精神科。对象:实验于1999-01/2003-08在解放军第102医院完成。选取符合中国精神疾病分类和诊断标准第三版关于精神分裂症诊断标准的患者177例,来自不同省区汉族军人,年龄18～45岁,病程1个月～20年。方法:采集117例精神分裂症患者的血样进行聚合酶链反应检测,并比较不同省区正常对照人群5-羟色胺2A受体基因型频率的分布有何差异。应用常规苯酚氯仿法抽提DNA,目的DNA的聚合酶链反应扩增及电泳检测,5-羟色胺2A受体基因T102C基因片断扩增的引物序列:5’-TCTGCTACAAGTTCTGGCTT-3’,5’-CTGCAGCTTTTTCTCTAGGG-3’;采用50μL反应体系,DNA0.5μg,引物50pmol,TagDNA酶2U加dNTP至终浓度200μmol/L。主要观察指标:精神分裂症患者DNA电泳检测及5-羟色胺2A受体基因型频率分布。结果:5-羟色胺2A受体各基因型频率分布为:A1A1,0.07～0.33;A1A2,0.50～0.72;A2A2,0.17～0.29。基因型频率在不同地区的相关分析:A1A1,χ2=4.44,P=0.6171,P>0.05;A1A2,χ2=1.14,P=0.9422,P>0.05;A2A2,χ2=0.93,P=0.9857,P>0.05。结论:117例精神分裂症患者5-羟色胺2A受体基因T102C基因型频率的地理分布比较均一。

BACKGROUND： Now researches on psychiatric disease and molecular heredity are becoming more and more gradually because of the development of molecular genetics, but conclusions are controversial, and for the time being there are no reports about whether there are regional differences and ethnical differences in the distribution of genotype frequency of 5-hydroxytryptamine 2A receptor gene T102C. OBJECTIVE： Researches on the geographic distribution of genotype frequency of 5-hydroxytryptamine 2A receptor gene T102C of schizophrenics people. DESIGN： Sample survey and observation with schizophrenics selected as subjects. SETTING： Mental Department, Changzhou Peace Hospital. PARTICIPANTS： The experiment was completed in The No.102 Hospital of PLA from January 1999 to August 2003. Totally 177 people of Han nationality （Age： 18-45; Duration of illness： 1 month-20 years） from different provinces and autonomous regions of China in compliance with diagnostic standards of Psychiatric Disease Classification and Diagnosis Standards of China （The 3^rd version） were selected as the subjects. METHODS： The blood samples from 117 schizophrenics were given test of polymerase chain reaction （PCR）, and the distribution differences of genotype frequency of 5-hydmxytryptamine receptor gene of normal control population from different provinces and autonomous regions were compared. DNA were abstracted by means of phenol chloroform, amplification and cataphoresis test of polymerase chain reaction of target DNA, primer sequence of gene segment amplification of 5-hydmxytryptamine （5-HT） 2A receptor gene T102C： 5 ＇-TCT GCT ACA AGT TCT GGC TT-3＇, 5 ＇-CTG CAG CTT TTT CTC TAG GG-3＇; Reaction system of 50 μL, DNA 0.5 μg, Primer 50 pmol, TagDNA enzyme 2U were added with dNTP to the final concentration of 200 μmol/L. MAIN OUTCOME MEASURES： DNA cataphoresis test of schizophrenic and distribution of genotype frequency of 5-hydrox- ytryptamine 2A receptor gene. RESULTS： The distribution of genotype frequency of 5-hydroxytryptamine （5-HT） 2A receptor gene： A1A1, 0.07-0.03; A1A2, 0.50-0.72, A2A2, 0.17-0.29. Correlation analysis of genotype frequency in different regions： A1A1, X^2=4.44, P=0.617 1, P 〉 0.05 ;A1A2, X^2=1.14, P=0.942 2, P 〉 0.05; A2A2, X^2=0.93, P=0.985 7, P 〉 0.05. CONCLUSION： The geographic distributions of genotype frequency of 5- hydmxytryptamine 2A receptor gene T102C of schizophrenics of the Chinese Han nationality people were comparatively even.