摘要
目的研究白细胞介素-1β(IL-1β)和白细胞介素-6(IL-6)致痫过程中谷氨酸(Glu)和γ-氨基丁酸(GABA)在大脑皮质及海马内表达的变化,探讨IL-1β及IL-6在癫痫发病中的作用机制。方法大鼠随机分为生理盐水对照组I、L-1β组I、L-6组,侧脑室注射相应试剂120 min后观察大鼠行为变化,并采用免疫组织化学方法检测大脑皮质及海马内Glu及GABA的表达变化。结果动物行为学观察,生理盐水对照组无明显癫痫发作,IL-1β组I、L-6组发作程度达中度。免疫组织化学染色显示,在注射IL-1β或IL-6 120 min后,IL-1βI、L-6组Glu表达在大脑皮质及海马较对照组明显升高,GABA表达较对照组明显降低,差异显著。结论IL-1β或IL-6可能通过升高Glu含量并降低γ-氨基丁酸的含量参与促痫和致痫过程,从而使神经元兴奋性升高促进癫痫发作。
Objective The changes of Glutamic acid（Glu） and GABA immunoreaction in the cerebral cortex and hippocampus of rats with seizure induced by IL-1β and IL-6 were studied .To explore the mechanism of IL-1β and IL-6 in epilepsy. Methods Experimental rats were randomly divided into 3 groups： control group, IL-1β group, and IL-6 group. After intracerebroventricular injection of relevant reagents for 120 min, behaviour changes were observed, Glu and GABA were examined by means of immunohistochemistry in the cerebral cortex and hippocampus of rats. Results The behaviour observation indicated that no seizure happened in control group, seizure in middle degree was observed in IL-1β group and IL-6 group. Compared with in control group,the immunoreaction of Glu showed that the expression was significantly increased in IL-1β group and IL-6 bgroup, while GABA was obviously decreased after intracerebroventricular injection IL-1β or IL-6 at 120 min. Conclusion The machanism of that IL-1β or IL-6 particpated in promotion and abduction epilepsy may be through increasing Glu and decreasing GABA in the cerebral cortex and hippocampus. The nerve excitation is enhanced and then epilepsy occurred.
出处
《解剖学报》
CAS
CSCD
北大核心
2005年第6期586-590,共5页
Acta Anatomica Sinica
基金
湖北省自然科学基金(2001ABB142)资助项目
