摘要
目的探讨在中国北方汉族人群中TNF-α基因启动子区单核苷酸多态性(SNPs)及其单倍型是否与HBV感染结局相关联。方法以212例无症状HBV携带者和207例慢性乙肝患者为研究对象,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)和序列特异性引物-PCR(SSP-PCR)方法对TNF-α基因启动子区5个位点,进行基因分型,用EPI和EH等软件分析各位点等位基因、基因型、单倍型频率及其组间差异。结果TNF-α基因-238GG基因型和-863CC基因型是HBV感染后个体发生乙型肝炎慢性化的易感因素(P=0.05,P<0.01)。5个位点组成的单倍型GGCCT在慢性肝炎组的频率显著低于无症状携带组(P<0.05),单倍型GGCAT和GGTAT在慢性肝炎组的频率显著高于无症状携带组(P<0.05)。结论TNF-α基因启动子区多态性可能是影响HBV感染结局的重要宿主遗传因素之一。
Objective To explore whether TNF-α promoter single nucleotide polymorphisms（SNPs） is associated with the outcomes of HBV infection. Methods Two hundred and twelve chronic asymptomatic HBV carriers and 207 chronic hepatitis B patients were recruited in this case-controled study in Beijing of China. Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） and sequence specific primer-PCR（SSP-PCR） were used to detect the SNPs of five sites in TNF-α promoter （ - 238G/A, - 308G/A, - 857C/T, - 863C/A, - 1031T/C）. The frequency distributions of genotypes and haplotypes in different groups were analyzed by EPI and EH programs. Results TNF-α -238 GG genotype and TNF-α - 863 CC genotype are susceptible factors to persistence of hepatitis B after HBV infection （ P = 0.05, P 〈 0.01）. Haplotypic frequency of GGCCT （ - 238/- 308/- 857/- 863/- 1031 ） in chronic hepatitis B patients was significantly lower than that in asymptomatic carriers （ P 〈 0.05）, and frequencies of haplotype GGCAT or GGTAT in chronic hepatitis B patients were significanthy higher than those in asymptomatic carriers （ P 〈 0.05 ）. Conclusions TNF-α promoter polymorphisms are important genetic factors affecting outcomes of HBV infection.
出处
《基础医学与临床》
CSCD
北大核心
2005年第12期1147-1151,共5页
Basic and Clinical Medicine
基金
北京市科技计划重大项目(H020920020590)
