糖基化终产物和高糖对小牛主动脉血管细胞的损伤作用

Glycated end products and high glucose damage vessel cells in bovine avtery

目的研究糖基化终产物(AGEs)和高浓度葡萄糖对血管细胞的损伤及对其糖基化终产物受体(RAGE)表达的影响。方法分别用10 g/L糖基化终产物1、0 mol/L葡萄糖和两者的混合作用于牛主动脉血管细胞,利用生化方法测定细胞乳酸脱氢酶(LDH)、还原型谷胱甘肽(GSH)和NO2-/NO3-的含量,用MTT法测定细胞的活性。用CELL-ELISA和RT-PCR检测细胞RAGE的表达。结果与正常组(C)相比,高糖组(G)、AGEs组(A)和联合损伤组(G+A)对主动脉内皮细胞的生长有明显的抑制作用(P<0.01)和对平滑肌细胞的生长有明显的促进作用,主动脉血管细胞LDH的泄漏量明显增高,GSH和NO2-/NO3-的含量明显减少(P<0.01);且在联合损伤组的作用最强。同时,联合损伤组血管细胞的RAGE表达水平明显高于AGEs组和高糖组。结论AGEs比高浓度葡萄糖更具损伤血管细胞的作用,且AGEs与高糖联合作用对细胞的损伤最为明显,这种联合损伤的过程也是通过RAGE介导的。

Objective To explore the mechanism of advanced glycation end products, high glucose and combined effects of both involved in the process of damaging vessel endothelial cell and vessel smooth muscle cell as well as their influence on the expression of AGEs receptor. Methods The bovine vessel endothelial cell and vessel smooth muscle cell in cell culture were affected by 10 g/L AGEs, 10 mol/L glucose and combined of both. The effects on damaging of cells were investigated by MTr, while we detected the contents of LDH, GSH and NO. The expression of RAGE mRNA was analysed by RT-PCR and Cell-ELISA. Results Compared with control group（C）,high glucose group（G）,AGEs group（A） and combined group（G ＋ A） were found to inhibit the growth of vessel endothelial cell and increase the proliferation of vessel smooth muscle cell significantly. Moreover, they can increase the content of LDH and decrease that of GSH and NO in both cells. In addition, the expression of RAGE was much higher in AGEs group and combined group than those in norreal group and high glucose group. Conclusion AGEs and high glucose have significantly pathogenic effects on vessel cells. Furthermore, AGEs and combined of AGRs and high glucose level damaged more evidently vessel cells, which was mediated by RAGE.