地被月季‘Royal Bassino’高频再生体系的建立

High Efficiency of in Vitro Micropropagation and Regeneration for Ground Cover Rose'Royal Bassino'

以地被月季‘Royal Bassino’为试材,进行了组培快繁和外植体再生条件的研究。结果表明,以带有腋芽的茎段为外植体,MS中添加6-BA 1.5 mg/L和IBA 0.1 mg/L为最适诱导培养基,诱导出芽率100%;MS中添加6-BA 0.5 mg/L和IBA 0.05 mg/L可以获得最高增殖倍数(6.3);而添加IBA 0.01 mg/L的1/2MS为最佳生根培养基,生根率92%。试管苗经7 d驯化后,移栽成活率在99%以上。分别以叶柄、叶盘和茎段为外植体进行再生培养,以MS为基本培养基,叶柄在添加噻苯隆(TDZ)7μmol/L的诱导培养基中暗培养10 d后,转接到添加6-BA 0.5 mg/L的再生培养基中光照培养约20 d,可以获得57.1%的芽再生率。

This experiment was conducted to investigate in vitro micropropagation and regeneration of petiole, leaf disk and internodal segments in ground cover rose （ Rosa hybrida L. ） ＇ Royal Bassino＇. The resuits showed that using axillary as explants, the optimum induction medium with 100% of shoots rate was basal MS supplemented with 6-BA 1.5 mg/L and IBA 0. 1 mg/L; and the highest shoot proliferation rate of 6. 3 times was obtained on the medium of MS ＋ 6-BA 0. 5 mg/L ＋ IBA 0. 05 mg/L; and 1/2MS with IBA 0. 01 mg/L was the optimum shoot rooting medium with 92% rooting. After 7 d of acclimation, the plantlets were transplanted to a greenhouse, and 99% survivals were obtained. Using petiole as explants for regeneration culture, derived either from loose callus or through organogenesis, the rate of adventitious buds was reached to 57. 1% through following procedures： dark-induced initially for 10 d on the medium of MS ＋ thidiazuron （TDZ） 7 μmol/L, then cultured on the medium of MS ＋6-BA 0. 5 mg/L for about 20 d under photoperiod of 16/8 h. However, no successful regenerations of adventitious buds were observed in the culture using leaf disks and intemodal segments.