墨兰炭疽菌侵染过程与几丁质酶、β-1,3-葡聚糖酶活性的变化

Studies on Infection Processes of Colletotrichum gloeosporioides and Activities of Chitinase and β-1,3-glucanase in Leaves of Cymbidium sinense

研究了墨兰炭疽菌的侵染过程以及墨兰叶片中几丁质酶和β-1,3-葡聚糖酶活性的变化。结果显示,炭疽菌接种12 h后开始产生侵染丝侵入墨兰叶片的气孔腔,60 h之前炭疽菌菌丝在叶肉细胞的胞间隙蔓延,从84 h开始,炭疽菌次生菌丝侵入叶肉细胞内,部分叶肉细胞解体。几丁质酶、β-1,3-葡聚糖酶活性在接种炭疽菌后缓慢上升,到84 h达到最大。表明炭疽菌侵入墨兰叶肉细胞的胞间隙和胞质中对几丁质酶和β-1,3-葡聚糖酶活性高峰的出现有重要影响。

The infection processes of Colletotrichum gloeosporioides and activities of chitinase and β-1, 3- glucanase in leaves of Cymbidium sinense were studied after inoculating C. gloeosporioides. The results showed that the stomatic cavities in the epicuticula of leaves of C. sinense were infected by the hyphae of C. gloeosporioides at 12 h after inoculation. Both of the primary and secondary hyphae of C. gloeosporioides extended and developed in the intercellular spaces of mesophyll tissues in 36 -60 h after inoculation. The cytoplasms were infected at 84 h, and disintegrated partly. The activities of chitinase and β-1, 3-glucanase increased inoculated with C. gloeosporioide. Both enzymes activities reached the highest level at 84 h. Therefore, the results suggest the importance of hyphae successfully infecting intercellular spaces and cytoplasm of mesophyll tissues in leaves of C. sinense as the occurrences of active peaks of chitinase and β-1, 3-glucanase.