基质金属蛋白酶-7基因在大肠癌组织中的表达及其意义

Expression of MMP-7 mRNA in human colorectal carcinoma tissues and its clinial significance

目的:探讨基质金属蛋白酶-7(matrixmetalloproteinase,MMP-7)基因在大肠癌中的表达与大肠癌Dukes分期及病理分型的关系。方法:用逆转录聚合酶链式反应(Reversetranscriptionpolymerasechainreaction,RT-PCR)检测MMP-7mRNA在64例大肠癌中的表达,并与相应的Dukes分期及病理分型进行对照。结果:MMP-7mRNA在大肠癌DukesA、B、C+D期的表达率分别为62·5%、91·7%、95·0%,不同分期之间阳性率差异有显著意义(P<0·05)。在大肠癌高分化、中分化、低分化+未分化癌中的表达率分别为63·6%、92·3%、96·3%,不同分型之间阳性率差异有显著意义(P<0·05)。结论:MMP-7mRNA有可能作为大肠癌侵袭、复发的标志物,作为判断预后的危险因素。

Objective：To investigate the relation between matrix metalloproteinase-7 mRNA expression in colorectal carcinomas with Dukes stages and pathological grades. Methods：RT-PCR technique was used to detect the expression of MMP-7 mRNA in 64 colorectal carcinoma tissues and compare with the Dukes stages and the pathological grades. Results：The expression rates of MMP-7 mRNA in different Dukes stages were 62. 5% , 91.7% and 95.0% respectively. The positive rate of MMP-7 mRNA in colorectal carcinomas was in significantly reverse proportion to Dukes stages （P〈0.05）. The expression rates of MMP-7 mRNA in different pathological grades were 63.6% , 92. 3% and 96. 3% respectively. The positive rate of MMP-7 mRNA in colorectal carcinomas was in significantly reverse proportion to pathological grades （ P〈0.05 ）. Conclusion ： MMP-7 mRNA may prove to be a marker for colorectal carcinomas in invasion and recurrence and may be a useful risk factor for the prognosis.