犬骨髓基质细胞和β-磷酸三钙复合物在裸鼠体内的成骨性能

Osteogenesis of the construct combined BMSCs with β-TCP in nude mice

目的:观察犬骨髓基质细胞(bone marrow stromalcells,BMSCs)和支架材料β-磷酸三钙(β-TCP)复合物植入裸鼠体内的成骨情况。方法:首先在体外构建BMSCs和β-TCP的复合物,大小3mm3左右。实验分为3组:裸鼠体内植入单纯β-TCP,植入未经体外培养的BMSCs和β-TCP复合体,植入体外培养5d的BMSCs和β-TCP复合体。植入后1周、1个月和3个月取出标本,进行大体观察、X线片观察、灰度测定、组织学观察及成骨图像分析。结果:植入后3个月,BMSCs+β-TCP体外培养组X线片密度最高,DigiDensX线片灰度值3组分别为:0.605>0.555>0.473(P<0.01)。HE染色显示:1个月后,BMSCs+β-TCP体外培养组见新骨和血管形成;3个月后,BMSCs+β-TCP体外培养组新骨形成明显,并有大量血管生成,BMSCs+β-TCP体外未培养组少量成骨,单纯β-TCP组未见明显成骨。成骨图像分析3组成骨密度均数分别为:23.99%、8.36%、1.23%(P<0.05)。结论:BMSCs+β-TCP复合物植入裸鼠体内4周出现新骨形成,3个月时,骨形成明显并有血管化生成。

PURPOSE： To study the osteogenic capability of the construct combined dog＇s bone marrow stromal cells （BMSCs） with β-tricalcium phosphate （β-TCP） in vivo. METHODS： 3 groups of materials were implanted into the nude mice .Group 1 （β-TCP with no cells）, Group 2 （β-TCP＋cells no incubated） and Group 3 （β-TCP＋cells incubated）, 1 week, 1 month and 3 months after implantation, the specimens were harvested for gross observation, radiographic examination, histological observation and KS400 osteogenous analysis. RESULTS： The group of β-BMSCs＋TCP incubated had the highest radiodensity. The DigiDens radiodensity of 3 groups were 0.605 （BMSCs＋β-TCP incubated）〉 0.555 （BMSCs＋β-TCP non-incubated）〉0,473 （β-TCP with no cells）, P〈0.01. HE staining showed the bone formation and vascularization 3 months later in the group of BMSCs＋β-TCP incubated. Bone formation in KS400 image analysis sequentially were, 23.99%, 8,36%, 1.23% with significant difference, CONCLUSIONS： Constructs of BMSCs with β-TCP have new bone formation, collagen synthesis and vascularization in nude mice implantation.