摘要
目的:探讨犬骨髓基质细胞(bone marrow stromal cells,BMSCs)体外复合预制三维下颌骨缺损仿真β-磷酸三钙(β-TCP)支架对犬下颌骨节段性缺损进行修复的可行性。方法:通过计算机辅助设计/制作和快速原型技术,制作3cm长犬下颌骨节段性缺损β-磷酸三钙支架,体外接种第3代骨髓基质细胞,培养5d天后回植,对犬一侧下颌骨节段性缺损进行修复,并设单纯材料作为对照组。术后1周、1个月、3个月观察局部伤口情况,术后1、3个月行X线片和三维CT成像,术后3个月将标本进行生物力学检测和组织学观察。结果:X线片观察显示,术后3个月,对照组缺损区低密度影,BMSCs+β-TCP组缺损区见高密度组织;三维CT成像显示术后3个月,对照组修复区吸收明显,达1/2以上;BMSCs+β-TCP组修复区未见明显吸收;生物力学检测显示,对照组和BMSCs+β-TCP组的牙槽嵴高度分别为9.16mm和18.54mm,最大弯曲载荷分别为42.90N和102.77N,应力分别为1.930N/mm2和3.504N/mm2,应变分别为54.50%和16.98%。经统计学处理,均有显著性差异;组织学观察显示术后3个月,BMSCs+β-TCP组成骨良好,支架周围可见多核巨细胞吞噬材料,并可见软骨形成区。结论:应用β-TCP三维支架复合犬BMSCs进行犬下颌骨节段性缺损修复存在一定的可行性。
PURPOSE: To study the possibility of the segmental mandible reconstruction using combined dog's bone marrow stromal cells (BMSCs) with 3-dimentional β-tricalcium phosphate (β-TCP) in vivo. METHODS: 3-dimentional mandible defect scaffold 3cm in length was fabricated using CAD/CAM and rapid prototyping. The incubated 3th generated cells were seeded with 3-D scaffold, the constructs (n=3) was implanted to repair the segmental mandible defect. The control group was scaffold (no cells, n=3) implantation alone. 1 week, 1 month and 3 months postoperatively, the dogs were taken X ray films and 3-D CT scanning. And 3 months postoperatively, the mandibles were harvested, examined, radiographed, and prepared for biomechanical examination and histological evaluation. ANOVA was used for statistical analysis (with SPSS10.0 software package). RESULTS: Radiograph displayed the higher radiodensity in group of BMSCs+β-TCP than in the control group; 3-D CT image showed defect of the control group absorbed nearly 2/3 more than the group of plus cells; The mechanical examination showed the average height of the mandible of the two groups were 18.54 mm and 9.16mm, the compression strength was 102.77N and 42.90N, the stress was 3.504N/mm^2 and 1.930 N/ mm^2 with significant difference; Histological examination showed new bone formation in the scaffolds in central sections of the defects in the experimental group 3 months later. CONCLUSIONS: The construct of 3-D scaffold seeded BMSCs ensured bone formation and vascularization throughout the procedure of segmental mandible defect reconstruction, closely resembling how tissue engineering would be used to reconstruct a mandible in the clinical setting.
出处
《中国口腔颌面外科杂志》
CAS
2005年第4期345-350,共6页
China Journal of Oral and Maxillofacial Surgery
基金
上海市重点学科(优势学科)建设项目(Y0203)
国家863计划子课题(2002AA205011)项目
上海市科委项目资助(05DJ14006)
上海市教委项目资助(04BC39)