UBAP1蛋白在鼻咽癌细胞中的表达和定位

Expression and location of UBAP1 protein associated with nasopharyngeal carcinoma

目的:研究泛肽相关新基因编码产物的特性和在鼻咽癌细胞内定位与表达。方法:利用生物信息学分析编码蛋白的一般性质并预测其定位,构建增强型绿色荧光蛋白(enhance green fluorescent prote in,EGFP)与UBAP1融合基因的真核表达载体pEGFP-C2-UBAP1,通过脂质体介导转染人鼻咽癌细胞系HNE1,荧光显微镜观察UBAP1基因编码蛋白在HNE1活细胞内定位。结果:融合蛋白可在HNE1细胞中高表达,主要定位在细胞核,有较为明显的核膜聚集现象。结论:UBAP1基因编码产物在HNE1中的表达差异可能是NPC发生的原因之一。

Objective To analyze the expression and location of coding protein of UBAP1 gene and to understand the relationship between the expression pattern of the protein and cell carcinogenesis. Methods Bioinformatics was used to analyze the protein character to provide an available clue of subsequent research. The codon frame cDNA was amplified by PCR, and subcloned into enhance green fluo- rescence protein （EGFP） of pEGFP-C2. The recombinant plasmid was transfected into HNE1 cells. The expression of coding protein was observed by fluorescence microscopy. Results The expressed GFP-fu- sion protein generated striking green fluorescence in the cytoplasm in HNE1 cells. EGFP/UBAP1 was expressed and existed mainly in the nuclear, especially accumulated on the nuclear envelope. Conclusion The expression difference in HNE1 might be related to the carcinogenesis of NPC.