糖尿病肾病患者AOPP与SOD,GPx,NPT的关系

Advanced oxidation protein products in diabetic nephropathy patients and its relation with superoxide dismutase,glutathione peroxidase,neopterin

目的:研究糖尿病肾病患者血清蛋白氧化损伤指标晚期蛋白氧化产物(AOPP)的改变及其与超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)及新喋呤(NPT)的关系,探讨蛋白氧化损伤与糖尿病肾病氧化应激状态和慢性炎症状态的关系。方法:糖尿病患者85例,根据尿微量白蛋白排泄率和肌酐水平分为无糖尿病肾病(DM)组、早期糖尿病肾病(DN3)组、临床糖尿病肾病(DN4)组、终末期糖尿病肾病(DN5)组。用W ikto-sarsat介绍的方法改进后分别测定各组的血清AOPP,黄嘌呤氧化酶法测定SOD,[5,5′-二硫代双(2-硝基苯甲酸)](DTNB)显色法测定GPx,ELISA方法测定血清NPT。结果:DN5组患者的血清AOPP[(117.8±64.8)μmol/L]和DN4组的血清AOPP[(80.0±23.0)μmol/L]显著高于DM组[(58.2±17.7)μmol/L](P<0.01)。DN3组血清AOPP[(72.7±17.2)μmol/L]与DM组间无显著差异。血清AOPP与SOD,GPx呈负相关(r分别为-0.217和-0.374,P<0.05),并与NPT呈正相关(r=0.499,P<0.01)。结论:糖尿病肾病患者的血清蛋白氧化较无糖尿病肾病患者增强,并且与糖尿病肾病氧化应激状态和慢性炎症状态有关。

Objective To determine the changes of advanced oxidation protein products （AOPP） in diabetic nephropathy （DN） patients, as well as its relationship with superoxide dismutase （SOD）, glutathione peroxidase （GPx） and neopterin （NPT）. Methods By the concentration of urinary albumin excretion rate （UAER） and creatinine （Cr）, 85 groups as non-DN group （DM）, early-staged DN staged DN group（DN5）. The concentration of the diabetes mellitus （DM） patients were divided into 4 group （ DN3 ） , significant DN group （DN4） and endserum AOPP was measured by ameliorated method introduced by Wikto-Sarsat, while SOD by Xanthine oxidase test, GPx by [5,5＇Dithio-bis （2-Nitrobenzoic aicd） ] （ DTNB ） reaction test and NPT by ELISA. Results AOPP in Group DN5 [（117.8±64.8 ）μmol/L] and Group DN4 [（80.0±23.0）μmol/L] were significantly higher than those in Group DM [（58.2±17.7）μmol/L]. There was no significant difference of AOPP between Group DN3 [（72.7±17.2）μmol/L] and Group DM. Serum AOPP negatively correlated with SOD and GPx（r=-0. 217 and -0. 374 respectively,P 〈0.05 ）, while positively correlated with NPT （ r=0. 499 ,P 〈0.01 ）. Conclusion DN patient has enhanced protein oxidation than DM patient, which is related to oxidative stress and chronic inflammation in DN.