白细胞介素10对脑缺血大鼠的神经保护作用

Neuroprotective effect of interleukin-10 in rats with cerebral ischemia

目的:观察白细胞介素10对大鼠缺血性脑损伤的保护作用,并比较侧脑室和尾静脉注入两种给药方式的效果。方法:实验于南方医科大学附属南方医院神经内科实验室内进行。①将60只清洁级SD大鼠随机分为3组(n=20),所有大鼠均采用三氯化铁化学诱导法制作大脑中动脉血栓闭塞模型,造模4h后进行神经症状评分(评分越低提示脑损害越轻,越高提示脑损害越严重)。②评分后侧脑室注射组大鼠右侧侧脑室注射20mg/L的白细胞介素101μg/kg,注射速度为15μg/h;尾静脉注射组大鼠尾静脉注射20mg/L白细胞介素101μg/kg,注射速度为15μg/h;对照组大鼠右侧侧脑室注射等体积生理盐水。③给药后24h再次进行神经症状评分,然后断头处死大鼠取脑,测量各组大鼠脑梗死范围。结果:60只大鼠进入结果分析。①脑梗死范围:侧脑室注射组和尾静脉注射组均显著小于对照组[(2.47±0.51)%,(2.43±0.62)%,(3.29±0.78)%,P<0.01],但两组间无差异。②神经症状评分:给药前3组无差异(P>0.05),给药后侧脑室注射组和尾静脉注射组均显著低于对照组(2.32±0.72,2.43±0.62,3.29±0.78,P<0.01),但两组间无差异。结论:无论侧脑室还是尾静脉注入白细胞介素10均可显著缩小急性缺血性大鼠的脑梗死面积并减轻脑梗死症状,两种给药方式无差异。提示白细胞介素10对大鼠脑缺血有明显的神经保护作用。

AIM： To observe the protective effect of interleukin-10 on ischemic brainin jury of rats, and compare the effects of lateral ventricle administration with caudal vein administration. METHODS： The experiment was carried out in the Laboratary of Neurology, Affiliated Nanfang Hospital, Southern Medical University. （1）Sixty SD rats of clearing grade were randomly divided into 3 groups （n=20）. All rats were made into models of thrombus emphraxis in middle cerebral artery （MCA） with ferric chloride chemical derivation. Nerve-symptom scores were evaluated 4 days after modeling （lower score suggested slight brain lesion, higher score suggested heavy brain lesion）. （2） After scores were evaluated, rats in the lateral-ventricle injection group were injected with 20mg/L interleukin-10 （1μg/kg） through the right lateral ventricle at the injecting rate of 15μg per hour; Rats in the caudal-vein injection group were injected with 20 mg/L interleukin-10 （1μg/kg） through the caudal vein at the injecting rate of 15 μg per hour; Rats in the control group were injected with normal saline of the same volum through the right lateral ventricle （3）Scores of nerve symptoms were re-evaluated 24 hours after administration, then rats were killed to take out brain tissue, and cerebral infarction area in rats were measured. RSULTS： Sixty rats were involved in the analysis of results. （1）Cerebral infarct area： It was significantly smaller in the lateral-ventricle injection group and the caudal-vein injection group than that in the control group [（2.47~0.51 ）%, （2.43~0.62）%, （3.29~0.78）%, P〈0. 01], and there was no difference between the former two groups. （2）Nerve-symptom scores： There was no difference among the 3 groups before administration （P〉 0.05）. After administration, it was obviously lower in the lateral-ventricle injection group and the candal-vein injection group than that in the control group （2.32±0.72,2.43±0.62,3.29±0.78, P 〈 0.01）,and there was no difference between the former two groups. CONCLUSION： Injection of interleukin-10 through both lateral ventricle and caudal vein can significantly decrease the infarction area of rats with acute cerebral isehemia, and there is no difference between the two administration ways, which indicates that interleukin-10 has a significant neuroproteetive effect on cerebral ischemia of rats.