中药脊髓康抗脊髓损伤作用实验

An experiment on the effect of Chinese herb Jisuikang against spinal injury

目的:观察中药脊髓康对实验性脊髓损伤大鼠神经功能恢复的影响,了解其作用是否有剂量依赖性,并与地塞米松进行阳性对照。方法:取SD大鼠60只随机分为6组(n=10)。①给药:地塞米松组大鼠灌胃给予地塞米松0.6g/kg;脊髓康12.5,25,50g/kg组灌胃脊髓康(由黄芪、当归尾、赤芍、地龙、川芎、桃仁、红花等组成,生药10.3g/g浸膏)12.5,25,50g/kg;假手术组和模型组给予等量的生理盐水。给药体积均为20mL/kg,连续给药5d。②造模:于末次给药后30min,用AllenWD法打击大鼠T9～l0造成大鼠脊髓中度损伤(10g×25mm),假手术组不打击。③各组大鼠分别于术后1,3,7,14d进行爬板试验并采用改良的Tarlov标准进行肌力测定,以术前肌张力为5分;测定后进行大鼠脊髓病理组织学检查。结果:60只大鼠全部进入结果分析。①爬板实验:脊髓康3个剂量组大鼠从第7天爬坡能力开始恢复,7,14d与模型组比较有明显差异(P<0.05)。地塞米松组大鼠从第3天开始恢复。②双后肢肌力:脊髓康3个剂量组和地塞米松组大鼠从第3天开始恢复,3,7,14d与模型组比较有明显差异(P<0.05)。③病理检查结果:地塞米松组与脊髓康50g/kg组大鼠脊髓组织出血、坏死程度较轻,各种病理变化较轻,边缘粘连不明显。结论:①12.5～50g/kg脊髓康均能促进脊髓损伤大鼠神经功能的恢复。②50g/kg脊髓康能防止脊髓损伤的继发性改变,促进脊髓组织的恢复,促进神经组织的再生。其效果与地塞米松相似。

AIM： To explore the effect of Jisuikang, the compound Chinese drug on neural functional restoration of rats with experimental spinal cord injury and understand whether has dose-dependent effect or not, and perform positive control with desamethasone. METHODS： Sixty SD Rats were separated randomly into six groups （n=10）. （1） The rats in the desamethasone group were given with 0.6 g./kg desamethasone with gastric perfusion. Those in the Jisuikang 12.5,25 and 50 g/kg group were treated with 12.5,25 and 50 g/kg Jisuikang （composed of membranous milkvetch root, Chinese angelica, red peony root, earth-worm, Szechwan lovge, rhizome, peach seed and safflower, etc., extracting with 10.3 g/g raw drug） with gastric perfusion. The rats in the sham operation group and model group were given saline of the same volume. Volume of administration was all 20 mL/kg for 5 days. （2） Building models： After the last administration for 30 minutes, the T9-10 of rats was beaten with Allen WD method to make moderate injury of spinal cord in rats （10 g×25 mm）. These in the sham operation group were not being hit. （3） Oblique board experiment was performed at the 1^st. 3^rd, 7^th and 14^th days in rats of every group, respectively after operation. Testing of muscle strength was conducted with modified Tarlov standard, and muscular tension before operation was 5 points. Spinal cord histopathologic examination was done after determination. RESULTS： Sixty rats were all involved in the result analysis. （1） Oblique board experiment： Ability of scratching board recovered from the 7^th day in the rats of 3 dose of Jisuikang group. At the 7^th and 14^th days there was significant difference as compared with the model group （P〈0.05）. It recovered from the 3^rd day in the rats of the desamethasonc group. （2） Muscle strength of double posterior limb： The rats recovered from the 34 day in the 3 doses of Jisuikang group and the desamethasone group. There was significant difference as compared with the model group at the 34, 7^th and 14^th days （P〈0.05）. （3） Results of pathological check： Hemorrhage and necrosis of the spinal cord tissue in rats from desamethasone group and the 50 g/kg Jisuikang group were weak, various pathological changes were weak and the adherence of margin was insignificant. CONCLUSION： （1） 12.5-50 g/kg Jisuikang can accelerate the recovery of neural function of rats with spinal injury. （2） 50 g/kg Jisuikang can prevent secondary changes of spinal cord injury and improve the recovery of spinal cord tissue and the regeneration of neural tissue, whose effect is similar to that of desamethasone.