尿路斑块蛋白Ib启动子启动Smac表达的膀胱癌特异性及活性

The Bladder Cancer-Specificity and Activity of the Expression of Smae Gene Promoted by UPIb Promoter

目的：研究尿路斑块蛋白Ib启动子启动Smac表达的膀胱癌特异性及活性。方法：采用RT-PCR和免疫组化方法分别检测膀胱癌细胞株BIU-87和其他多种非膀胱癌细胞株在转染含尿路斑块蛋白Ib启动子和Smac基因的真核表达质粒pcDNA3-UpIb promoter-Smac前后Smac mRNA和蛋白质的表达变化。结果：BIU-87在转染后Smac mRNA的表达比转染前增强约2．1倍,Smac蛋白表达阳性细胞率也由转染前的约25．6％增加为转染后的约70．5％；非膀胱癌细胞株转染前后SmacmRNA及蛋白的表达没有显著性变化。结论：尿路斑块蛋白Ib启动子启动Smac表达具有膀胱癌靶向性及相当启动活性,为膀胱癌的靶向基因治疗研究奠定了基础。

Objective： To investigate the bladder cancer-specificity and activity of the expression of Smac gene promoted by UpIb promoter. Methods： Smac mRNA and protein were detected in BIU87 cell line and many other non-bladder cancer cell lines before and after transfection with pcDNAB-UpIb promoter-Smac including UpIb promoter and Smac gene by RT-PCR and immunohistochemical method, respectively. Results： The expression of Smac mRNA in BIU87 cell line increased about 2.1 times after transfection. The Smac protein positive cell rate of BIU-87 cell line was about 25.6% before transfection and it increased to about 7015% after transfection. There was no significant difference about the expression of Smac mRNA and protein in non-bladder cancer cell lines before and after transfection, Conclusions： UpIb promoter could promote the expression of Smac gene with bladder cancer-specificity and considerable activity, which laid the foundation of target gene therapy for bladder cancer.