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HSPgp96体外诱导腹腔巨噬细胞抗肿瘤细胞毒作用 被引量：1

The Cytotoxic Effect of Peritoneal Elicited Macrophages Induced by HSPgp96 on Anti-Tumor in vitro
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摘要 目的：探讨HSPgp96对小鼠PEMφ体外抗肿瘤细胞毒作用的影响。方法：分离巯基乙酸盐诱导的小鼠PEMφ,随机分为培养液对照组、LPS诱导组、HSPgp96诱导组。应用硝酸还原酶法、MTT法、扫描电镜分别观测PEMφNO的生成、对肝癌细胞H22的细胞毒作用及形态学变化。结果：在HSPgp96的体外诱导下,小鼠PEMφNO的生成量显著增加,对肝癌细胞H22的细胞毒作用明显增强,其效应与LPS相当。结论：HSPgp96可在体外有效激活PEM中的抗肿瘤细胞毒作用,NO是PEMφ杀瘤机制中的重要效应分子之一。 Objective： To evaluate the cytotoxic effect of PEMФ induced by HSPgp96 on anti-tumor in vitro. Methods： PEMФ separated from mice induced by thioglycolate were divided into three groups randomly： Culture medium in control; LPS-induced group; HSPgp96-induced group. The production of NO, the cytotoxic effect to H22 cells and the morphologic change of PEMФ were investigated separately by enzyme method, MTT assay and scanning electron microscope. Results： In vitro, HSPgp96 can increased NO production from PEMФ of mice and significantly enhance the cytotoxic effect of PEMФ to H22 cells as well as LPS. Conclusion： HSPgp96 can effectively induce the cytotoxic effect of PEMФ on anti-tumor in which NO is one of the capital effective molecules in vitro.
作者 施海燕 顾君一 张天一 林琳 朱昌来
机构地区 南通大学基础医学院病理生理学教研室 南通大学神经再生重点实验室
出处 《中国肿瘤生物治疗杂志》 CAS CSCD 2005年第4期272-274,共3页 Chinese Journal of Cancer Biotherapy
基金 江苏省教育厅基金(03KJD320183)
关键词 热休克蛋白GP96 腹腔巨噬细胞 肿瘤 细胞毒作用 heat shock protein gp96 peritoneal elicited macrophages tumor cytotoxicity
分类号 R730.51 [医药卫生—肿瘤]
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参考文献7

二级参考文献49

  • 1[1]Linderoth NA, Popowicz A, Sastry S. Identification of the peptidebinging sites in the heat shock chaperone/tumor rejection antigen gp96 (grp94)[J]. J Biol Chem, 2000, 275(8): 5472-5477.
  • 2[2]Robert J, Menoret A, Cohen N. Cell surface expression of the endoplasmic reticular heat shock protein gp96 is phylogenetically conserved[J]. J Immunol, 1999, 163: 4133-4139.
  • 3[3]Heike M, Noll B, Buschenfelde KHM. Heat shock protein-peptide complexes for use in vaccines[ J]. J Leukoc Biol, 1996, 60: 153-158.
  • 4[4]Wang XY, Kaneko Y, Repasky E, et al. Heat shock proteins and cancer immunotherapy[J]. Immunol Invest, 2000, 29(2): 131-137.
  • 5[5]Arnold D, Wahl C, Faath S, et al. Influences of transporter associated with antigen processing (TAP) on the repertoire of peptides associated with the endoplasmic reticulum-resident stress protein gp96[J]. J Exp Med, 1997, 186(3): 461-466.
  • 6[6]Menoret A, Peng P, Srivastava PK. Association of peptides with heat shock protein gp96 occurs in vivo and not after cells lysis[J].Biochem Biophys Res Commun, 1999, 262: 813-818.
  • 7[7]Ishii T, Udono H, Yamano T, et al. Isolation of MHC class I-restricted tumor antigen peptide and its precursors associated with heat shock proteins hsp70, hsp90, and gp96 [ J ]. J Immunol,1999, 162: 1303-1309.
  • 8[8]Blachere NE, Li Z, Chandawarkar RY, et al. Heat shock proteinpeptide complexes, reconstituted in vitro, elicit peptide-specific cytotoxic T lymphocyte response and tumor immunity[J]. J Exp Med, 1997, 186(8): 1315-1322.
  • 9[9]Sastry S, Linderoth N. Molecular mechanisms of peptide loading by the tumor rejection/heat shock chaperone gp96 (grp94)[ J ]. J Biol Chem, 1999, 274(17): 12023-12035.
  • 10[10]Amold-Schild D, Hanau D, Spehner D, et al. Cutting edge: Receptor-mediated endocytosis of heat shock proteins by professional antigen-presenting cells[J]. J Immunol, 1999, 162: 3757-3760.

共引文献11

同被引文献16

  • 1杨峰,万涛,周向阳,吴艳峰,李楠,陈国友,蒋应明,曹雪涛.HSP70L1与OVA融合蛋白的制备及其生物学功能的初步研究[J].中国肿瘤生物治疗杂志,2006,13(2):129-134. 被引量：2
  • 2Lee-Yoon D, Easton D, Murawski M, et ah Identification of a major subfamily of large Hsp70-like proteins through the cloning of the mammalian 110-kDa heat shock protein. Biol Chemi, 1995, 270 ( 26 ) : 15725-15733.
  • 3Chen X, Easton D, Oh HJ,et al. The 170kDa glucose regulated stress protein is a large Hsp70-, Hsp110-like protein of the endoplasmic reticulum. FEBS Lett, 1996, 380(1-2) :68-72.
  • 4Oh HJ, Easton D, Murawski M, et al. The chaperoning activity of Hsp110:identification of functional domains by use of targeted deletions. J Biol Chem, 1999,274(22):15712-15718.
  • 5Hwang TS, Han HS, Choi HK, et al. Differenial,stage-dependent expression of Hsp70, Hsp110 and Bcl-2 in colorectal cancer Res. J Gastroenterol Hepatol,2003, 18 ( 6 ) : 690 -700.
  • 6Manjili MH,Henderson R,Wang XY,et al. Development of a recombinant Hsp110-HER-2/neu vaccine using the chaperoning properties of Hsp110. Cancer Res,2002, 62 (6) : 1737-1742.
  • 7Manjili MH,Wang XY, Chen X, et al. Hsp110-Her2/neu chaperone complex vaccine induces protective immunity against spontaneous mammary tumors in Her-2/neu transgenic mice. J Immunol, 2003, 171 (8) :4054-4061.
  • 8Manjili MH,Park J,Facciponte JG,et al. HSP110 induces "danger singals" upon interaction with antigen presenting cells and mouse mammary carcinoma. Immunobiology,2005,210 ( 5 ) :295-303.
  • 9Wang XY, Arnouk H, Chen X, et al. Extracellular targeting of endoplasmic reticulum chaperone glucose-regulated protein 170 enhances tumor immunity to a poorly immunogenic melanoma. J Immunol , 2006, 177(3) :1543-1551.
  • 10Schijns VE. Immunological concepts of vaccine adjuvant activity. Curr Opin Immunol,2000,12 ( 4 ) :456-463.

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中国肿瘤生物治疗杂志
2005年 第4期

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