甘草中差向异构体对D—氨基半乳糖致大鼠肝损伤治疗作用的肝组织学和亚微结构研究

Curative Effects of Epimeric Glycyrrhizic on Hepatic Injury Induced by D-galactosamine in Rat: Histological and Submicrostructure Research

大鼠腹腔注射D—氨基半乳糖450mg.kg^(-1),引起急性肝损害,同时分别给予甘草差向异构α体和β体300ms.kg^(-1),通过光学显微镜和透射电镜观察肝组织学和肝亚微结构的变化。显示α体能明显减轻D—氨基半乳糖中毒24小时、48小时引起的肝组织和肝亚微结构的病变,而β体只能减轻中毒24小时大鼠的病变,对中毒48小时大鼠无影响。提示。体抗D—氨基半乳糖致大鼠肝损害作用优于β体。

After inducing acute hepatic injury through intraperitoneal injection of D-galactosamine 450mg ?kg-1 in rat, 18 α-form and 18 β-form epimeric glycyrrhizic acid monoammonium salt dihydrate 300mg ?kg-1 and same volume of physiological saline were respectively administered to four groups of rats which were 18 α-form group, 18 β-form group, control group of hepatic injury and control group of normality, and the hepatic pathological changes in rats were observed by light and electron transmission microscope. The results showed that 18 α-form epimeric glycyrrhizic acid can remarkably alleviate hepatic lesion in. rats after intoxicating for 24 to 48 hours, and 18 β-form epimeric glycyrrhizic acid had not effects to rats after intoxicating 48 hours. It is considered that 18 α-form epimeric glycyrrhizic acid is more effective than 18 β-form on antihepatic injury in rat.