摘要
目的:研究氯桂丁胺对小鼠细胞色素P450(CYP450)含量的影响及与大鼠血浆蛋白结合率。方法:用分光 光度法测定CYP450含量,用平衡透析法测定蛋白结合率。结果:氯桂丁胺对小鼠肝脏CYP450酶总含量没有影响,氯桂 丁胺在血浆中的浓度为4-8μg/ml时,与大鼠血浆蛋白结合率为71.79%-73.56%,不同剂量组间药物与血浆蛋白的结 合率差异无显著性(P>0.05)。结论:氯桂丁胺对肝药酶无诱导或抑制作用,属中等蛋白结合率的药物。
Objective:To investigate the influence of DCPB (3,4 - dichlorophenyl - propenoyl - sec. - butylamine) on the content of cyp450 in mice and the plasm protein binding rate. Methods : the binding rate of DCPB to plasma protein was determined by equilibrium dialysis method, and the content of CYP450 was assayed by differential spectrum method. Results :The resuits showed that DCPB has no effect on the total content of CYP450 in mice, and the binding rate of DCPB to plasma protein was found to be 71.79% - 73.56% when the concentration of DCPB in plasma was 4 - 8μg/ml. There is no significant difference in the binding rate between different dose groups ( P 〉 0. 05 ). Conclusion : CYP450 was not induced or inhibited by DCPD whose protein binding rate is medium.
出处
《包头医学院学报》
CAS
2005年第4期339-341,共3页
Journal of Baotou Medical College