氯桂丁胺对小鼠CYP450含量的影响及与大鼠血浆蛋白结合率

Effect of 3,4 - dichlorophenyl - propenoyl - sec. - butylamine on the Content of Cyp450 in Mice and Plasma Protein Binding Rate in Rats

目的:研究氯桂丁胺对小鼠细胞色素P450(CYP450)含量的影响及与大鼠血浆蛋白结合率。方法:用分光 光度法测定CYP450含量,用平衡透析法测定蛋白结合率。结果:氯桂丁胺对小鼠肝脏CYP450酶总含量没有影响,氯桂 丁胺在血浆中的浓度为4-8μg/ml时,与大鼠血浆蛋白结合率为71．79％-73．56％,不同剂量组间药物与血浆蛋白的结 合率差异无显著性(P>0．05)。结论:氯桂丁胺对肝药酶无诱导或抑制作用,属中等蛋白结合率的药物。

Objective：To investigate the influence of DCPB （3,4 - dichlorophenyl - propenoyl - sec. - butylamine） on the content of cyp450 in mice and the plasm protein binding rate. Methods ： the binding rate of DCPB to plasma protein was determined by equilibrium dialysis method, and the content of CYP450 was assayed by differential spectrum method. Results ：The resuits showed that DCPB has no effect on the total content of CYP450 in mice, and the binding rate of DCPB to plasma protein was found to be 71.79% - 73.56% when the concentration of DCPB in plasma was 4 - 8μg/ml. There is no significant difference in the binding rate between different dose groups （ P 〉 0. 05 ）. Conclusion ： CYP450 was not induced or inhibited by DCPD whose protein binding rate is medium.