国产膨体聚四氟乙烯植入大鼠皮下后的相关组织学变化

Histological changes of domestic e-PTFE implanted into subcutenous tissue of rat

目的探讨经不同方法处理后的国产膨体聚四氟乙烯软组织补片植入实验动物皮下后的相关组织学变化,为临床应用提供参考依据。方法利用组织学和组织化学技术观察在动物皮下植入经不同条件处理的膨体聚四氟乙烯与周围组织相嵌的动态变化及其本身材料的变化。结果对照组和不同方法处理后的膨体聚四氟乙烯植入后均有组织长入。植入后3d对照组细胞浸润量最大,至28d的组织长入量最多,其次为切削组。高压高温组和钳夹组细胞浸润量和组织长入量较少,并有明显统计学差异。结论动物体内移植国产膨体聚四氟乙烯软组织补片后72h即有细胞浸润,植入后7d即有明显的组织长入。膨体聚四氟乙烯软组织补片不同的处理方法对组织长入时间的影响可供临床应用参考。

Objective To investigate the relative histological change of transplanted domestic expanded polytetrafluoroethylene （e-PTFE）, which were treated with different methods, in order to offer the.referential data for clinical application. Methods e-PTFE treated with different methods was transplanted into subcutaneous tussue of rat. The samples harvested according to time sequence were examined by using histological and histoehemical methods, Dynamic change of the structure between the e-PTFE and it＇s surrounded tissue was investigated based on the examination. Results Cell and tissue were observed on the inside of all the e-PTFE including the control group and the experiment groups. Heavy cell infiltration on the 3rd day was the most significant in control group, and the quantity of tissue ingrowth was also the most until the 28th day. The next was trimming group. The quantity of both cell infiltration and the tissue ingrowth in high pressure steamed group and forceps squeezed group were less than that in other groups. Conclusion Cell infiltration into domestic e-PTFE is significantly achieved since 72 hrs and fibrovascular ingrowth since the 7days after implanted e-PTFE under subcutaneous tissue of the rat. Different treatment methods of e-PTFE can affect the speed of tissue ingrowth into the e-PTFE, which could be a reference for clinic application of e-PTFE.