TNFA-308基因多态性与中国人群慢性牙周炎的相关性研究

Association of TNFA-308 gene polymorphisms with susceptibility to chronic periodontitis in Chinese patients

目的:探讨TNFA-308基因多态性与慢性牙周炎易感性的关系。方法:收集63例重度慢性牙周炎(CP)患者、103例轻、中度慢性牙周炎患者及80例健康对照者的颊黏膜拭子,提取DNA,采用多聚酶链反应-限制性内切酶片段长度多态性(PCR-RFLP)法测定TNFA-308位点的基因多态性,实验结果输入SPSS10.0统计软件包处理,进行各组间基因型分布和等位基因频率的χ2检验,比较各组间基因型分布的差异。计算等位基因2的OR和95%可信区间,以确定等位基因2与牙周炎易感性的关系。结果:重度CP组、轻、中度CP组及健康对照组均以TNF1/1纯合子占优势,TNF1/2杂合子基因型次之,仅在轻、中度CP组检出1例TNF2/2纯合子基因型。TNFA-308/NcoI基因型分布及等位基因频率在3组间无显著性差异。结论:对此位点基因型是否对慢性牙周炎的易感性及进程预后产生影响,尚不能肯定,需要进一步的研究。

PURPOSE： The purpose of this study was to investigate the relationship between TNFα-308 gene polymorphisms and susceptibility to chronic periodontitis in Chinese patients. METHODS： DNA samples with buccal swabs were collected from 63 severe CP patients, 103 initial to moderate CP patients, and 80 healthy controls in Chinese HaM nationality. The TNFA-308 gene polymorphisms were analyzed with PCR-RFLP. The data were analyzed by X^2 test using SPSS10.0. RESULTS： There were no significant genotype distribution differences of TNFA-308 between either two of the three subject groups. CONCLUSION： Further study was needed since we did not find the relationship between TNFA-308 gene polymorphism and periodontitis in this present study.