青春期龈炎龈下菌斑中牙龈卟啉单胞菌的检测

Detection of Porphyromonas gingivalis in the subgingival plaque of puberty gingivitis

目的:检测青春期龈炎患者龈下菌斑中牙龈卟啉单胞菌(Porphyromonas gingivalis,Pg)的存在情况,探讨青春期Pg与牙龈健康状况的关系。方法:14～17岁青春期龈炎患者及牙龈健康者各36例,采用16SrRNA聚合酶链反应(polymerase chain reaction,PCR)检测2组受检者的龈下菌斑样本中Pg的存在,并用电泳凝胶成像分析软件检测各电泳条带的平均灰度值,计算Pg的相对含量;同时检测并记录受检者的各牙周指数测值,观察其与Pg相对含量的相关性。数据用SPSS11.5软件包作统计学分析。2组Pg阳性检出率的比较采用χ2检验;Pg的相对含量以及Pg阳性检出者与阴性检出者的牙龈炎严重度的比较采用Wilcoxon秩和检验;Pg的相对含量与各牙周指数之间的相关关系采用Spearman相关分析。结果:青春期龈炎组与牙龈健康组的龈下菌斑中Pg的阳性检出率分别为47.22%和25.00%,两者之间具有显著性差异(P<0.05);青春期龈炎组与牙龈健康组的Pg在龈下菌斑中的平均相对含量分别为48.02%和21.46%,统计学上有高度显著性差异(P<0.01);在青春期龈炎组Pg阳性个体中,Pg的相对含量与牙龈指数(gingivalindex,GI)、龈沟出血指数(sulcusbleedingindex,SBI)、探诊深度(probedepth,PD)数值的高低呈正相关;青春期龈炎组中,Pg阳性检出者的GI、SBI数值高于阴性检出者,两者之间具有高度显著性差异(P<0.01)。结论:青春期个体的龈下菌斑中有Pg的定植,且与牙龈健康状况有密切关系。

PURPOSE： To detect the presence of Porphyromonas gingivalis （Pg） in the subgingival plaque of puberty gingivitis and to investigate its relationship with gingival health. METHODS： The subjects of 14- to 17-year-old children in this study were divided into two groups, puberty gingivitis group and gingival healthy group, with 36 in each group. Periodontal parameters were recorded. A 16S rRNA-based polymerase chain reaction （PCR） detection method was used to determine the prevalence of Pg in the subgingival plaque samples. The AVG of positive band in the agarose gel electrophoresis was analyzed using the automatic gel documentation and image analyzer software. The relative quantity of Pg was determined. The data were statistically analyzed using SPSS11.5 software. Chi-square test was used to compare the prevalence of Pg in the two groups. Wilcoxon rank sum test was used to analyze the relative quantity of Pg and the severity between the Pg-positive and the Pg-negative subjects. Spearman correlation analysis was used to test the relationship between the relative quantity of Pg and periodontal parameters. RESULTS： The prevalence of Pg in the puberty gingivitis group and gingival healthy group was 47.22% and 25.00%, respectively （P〈0.05）. The relative quantity of Pg in the two groups was 48.02% and 21.46%, respectively（P〈0.01）. In all the Pg-positive subjects of puberty gingivitis group, there was positive correlation between the relative quantity of Pg and the gingival index （GI）,sulcus bleeding index （SBI） and the probe depth （PD）. There were statistically differences between them. The GI and SBI index of the Pg-positive subjects were statistically higher than that of the Pg-negative subjects in the puberty gingivitis group （P〈0.01）. CONCLUSION： Periodontal pathogens can colonize in the subgingival plaque in puberty subjects and colonization of Pg is relevant to the status of gingival health.