便秘型肠易激综合征结肠黏膜组织蛋白质组双向凝胶电泳分析

Proteomic analysis of colonic mucosa by two-dimensional gel electrophoresis in constipation-predominant irritable bowel syndrome

目的:分析便秘型肠易激综合征(C-IBS)患者与正常人结肠黏膜组织蛋白质表达的差异．方法:采用双向凝胶电泳(2-DE)技术和计算机辅助的图像分析方法,对C-IBS患者与正常人结肠黏膜组织蛋白质进行分离和比较分析．结果:正常对照组平均胶蛋白斑点数为308,C-IBS组平均胶蛋白斑点数为238,与正常对照组平均胶匹配点数为178,匹配率74．79％．有18个蛋白质点的表达量存在明显差异,其中有3个蛋白点表达发生明显上调,有15个蛋白点表达发生明显下调．结论:C-IBS患者与正常人结肠黏膜组织蛋白质表达存在明显差异,可能与C-IBS的发病机制有关．

AIM： To analyze the differential expression of proteome in colonic mucosa between patients with constipation- predominant irritable bowel syndrome （C-IBS） and the healthy controls. METHODS： Two dimensional polyacrylamide gel electrophoresis （2-DE） technique and computer-assisted image analysis were used to separate the protein spots and analyze the differential expression of proteome in the colonic mucosa of the healthy controls and patients with C-IBS. RESULTS： A total of 308 protein spots were identified in the av-erage gel of the healthy controls, and 238 in patients with C-IBS. A total of 178 protein spots were matched, and the mean matching rate was 74.49%. There were 18 protein spots that were significantly differentially expressed. Of those 18 protein spots, the expression of 3 spots were increased markedly, while 15 were decreased significantly. CONCLUSION： The proteomic expression in colonic mucosa of patients with C-IBS is significantly different from that of the healthy controls, which may be associated with the pathogenesis of C-IBS.