奥沙拉秦钠对溃疡性结肠炎一氧化氮合酶表达的影响

Effects of olsalazine sodium on expression of nitric oxide synthase in patients with ulcerative colitis: an analysis of 36 cases

目的:探讨奥沙拉秦钠对溃疡性结肠炎一氧化氮合酶表达的影响．方法:收集36例活动期患者结肠黏膜标本,应用免疫组化方法检测奥沙拉秦钠治疗前后内皮型一氧化氮合酶(eNOS)和诱生型一氧化氮合酶(iNOS)及硝基酪氨酸的表达．结果:4例患者炎症部位结肠黏膜上皮细胞表达eNOS,浸润炎症细胞无eNOS表达．炎症部位黏膜上皮细胞36例(100％) 表达iNOS,其中13例为强阳性,23例为弱阳性;1例在腺泡上皮细胞有弱阳性表达;18例(50%)固有层炎症细胞呈现 iNOS阳性．经奥沙拉秦钠治疗后,缓解期只有5例患者黏膜上皮细胞表达iNOS,为弱阳性．非炎症部位各类细胞均无 iNOS表达．5例患者在炎症部位黏膜上皮细胞中有硝基酪氨酸表达,其中1例伴腺泡上皮硝基酪氨酸表达,表达均为弱阳性,固有层浸润炎症细胞未见表达,非炎症部位各类细胞均无表达．结论:奥沙拉秦钠可抑制iNOS的产生,减轻炎症,促进溃疡愈合．

AIM： To explore the effects of olsalazine sodium on the expression of nitric oxide synthase （NOS） in patients with ulcerative colitis （UC）. METHODS： The colonic mucosa specimens were collected from 36 patients with active ulcerative colitis. The expression of inducible NOS （iNOS）, epithelial NOS（eNOS）, and nitrotyrosine were detected by immunohistochemistry before and after the patients were treated with olsalazine sodium. RESULTS： eNOS was expressed in the mucosal epithelial cells of the inflammatory area in 4 patients, but not expressed in the inflammatory cells, iNOS was expressed in the mucosal epithelial cells of all the 36 （100%） samples （13 strong positive and 23 light positive） taken from inflammatory area. iNOS light positive staining in acinar cells of colonic epithelium was detected in 1 patient only. Inflammatory cells in the lamina propria expressed iNOS in 18 （50%） cases. After the treatment of olsalazine sodium, iNOS was lightly and positively expressed in the mucosal epithelial cells of only 5 patients, and no iNOS was expressed in non-inflammatory areas. Nitrotyrosine was detected in the mucosal epithelial cells of inflammatory areas in 5 patients, of which only 1 was accompanied by light and positive expression of nitrotyrosine in the acinar epithelial cells. No nitrotyrosine expression was found in the inflammatory cells of the lamina propria and noninflammatory areas. CONCLUSION： Olsalazine sodium can inhibit the expression of iNOS, alleviate the inflammation, and promote the healing of the ulcer.