人偏肺病毒黏附蛋白的二级结构及B细胞表位初步预测

Prediction of the secondary structure and B cell epitopes of human metapneumovirus attachment protein

目的预测人偏肺病毒G蛋白的二级结构及B细胞表位.方法分别采用SOPMA方法及HMMTOP程序预测G蛋白的二级结构和跨膜区域;综合分析蛋白的柔性结构、亲水性、表面可及性与抗原性指数,预测G蛋白的抗原表位.结果G蛋白的二级结构主要为柔性区域,占62.1%;α-螺旋占22.37%;β-折叠占15.53%;N端第32～51位氨基酸残基为跨膜区.B细胞表位位于G蛋白N端55～77、80～104、111～126、130～167、178～210区段.结论应用多参数预测G蛋白的二级结构与B细胞表位,为进一步研究蛋白特征、单克隆抗体制备及表位疫苗研制奠定了基础.

Objective To predict the secondary structure and B cell epitopes of human metapneumovirns attachment（G） protein. Methods The secondary structure and transmembrane domain were predicted by SOPMA and HMMTOP respectively. Hydrophilicity profile, surface probability and antigenicity index predicted by methods of Kyte-Dcolitfle, Emini and Jameson-Wolf were combined and the possible B cell epitopes of G protein were predicted. Results The flexible regions were the main structure type, locating at the N-terminal No.27-31, 38-39, 56-77, 81-124, 131-146, 151-167, 180-188, 193-212 regions. There were a-helix regions at the N-terminal No. 5-26, 40-46, 125-130, 168-175, 213-218. And the N-terminal No. 1-4, 32-37, 47-55, 78-80, 147-150, 176- 179, 189-192 were the β-sheet regions. The N-terminal No.32-51 was the transmembrane domain. B cell epitopes were probably located at or adjacent the N-terminal No.55-77, 80-104, 111-126, 130-167, 178-210 regions, and the former three fragments were the predominant epitopes. Conclusion Prediction of the secondary structure and B cell epitopes of G protein lay the basis for studies of the characteristics of the protein, development of epitopebased vaccine and the monoelonal antibody against G protein.