β照射对NIH3T3细胞中基质金属蛋白酶和糖基转移酶表达的影响及牛磺酸的干预作用

Effects of β-rays on the expressions of MMP2 and PPGalNAcT2 in NIH3T3 cell lines and the interference of Taurine

目的观察β射线对NIH3T3成纤维细胞中基质金属蛋白酶2(MMP2)和N-乙酰氨基半乳糖转移酶2(ppGalNAcT2,E.C2.4.1.41)基因mRNA表达的影响,并探讨牛磺酸对β辐射损伤的防护作用。方法以不同剂量的高能电子射线模拟β射线照射NIH3T3细胞,另外在15Gy照射组中提前12h加入不同浓度的牛磺酸(Tau),照后12h收集各组细胞,采用RT-PCR法检测MMP2、ppGalNAcT2在mRNA水平的表达差异。结果β射线照射NIH3T3细胞后MMP2的mRNA表达量增加,且与照射剂量呈正相关,ppGalNAcT2的变化情况正好相反,加入牛磺酸后可以起干预作用。结论5～15Gyβ射线照射对NIH3T3细胞中ppGalNAcT2在mRNA水平的表达有抑制作用,提示ppGalNAcT2可能与细胞的癌变等过程有关,牛磺酸能抑制MMP2的表达,同时促进ppGalNAcT2的表达,因而具有辐射防护作用。

Objective To observe the effects of β-irradiation on the mRNA expressions of MMP2（ matrix metalloproteinase 2） and ppGalNAcT2 （polypeptide N-acetylgalactosaminyltransferase 2） in NIH3T3 cells, and to study the biologic protective effect of Tau（Taurine） for β-irradiation＇ s damage. Method After NIH3T3 cells （including Tan groups）were exposed to different doses of high energy electronic rays which mocked β-irradiafion, the expressions at mRNA level of MMP2 and ppGalNAcT2 were examined by reverse transcription polymerase chain reaction （RT-PCR） within 12 h. Results The results showed that the mRNA expression of MMP2 was increased evidently in NIH3T3 cells irradiated by β-irradiation, and it was positively related to the dosage. The changes of ppGalNAcT2 was Tan could interfere with the changes. The mRNA expression of ppGalNAcT2 in NIH3T3 cells could be inhibited by β-irradiation, and it is postulated that ppGalNAcT2 might be related to the progression of cell＇ s dissociation. Conclusion Tan can inhibit the expressions of MMP2, meanwhile stimulate the expression of ppGalNAcT2, so it has biologic protection effect at cell level.