高钙与高蛋白日粮诱发鸡痛风的尿液临床病理学研究

Clinicopathology of urine in growing layers with gout induced by high dietary calcium and high protein

将120羽35日龄伊沙蛋鸡随机均分为对照组、高钙组、高蛋白组和高钙高蛋白组,进行30 d的饲养试验。50 日龄时测定分析鸡的尿液和尿沉渣。结果表明:高钙高蛋白组鸡发生了典型的内脏型痛风;与对照组和高蛋白组相比, 高钙组和高钙高蛋白组鸡尿量显著增加,尿液pH值升高,尿液中钙、钠、钾和镁相对含量和24 h尿钠总量显著降低; 高钙组和高钙高蛋白组鸡尿液中尿酸、钙、无机磷、钾和镁的24 h总量和高蛋白组尿液无机磷的相对含量以及尿酸和无机磷的24 h总量显著高于对照组;对照组和高蛋白组鸡尿沉渣以球状晶体居多;高钙组鸡尿沉渣中以针状晶体较多;高钙高蛋白组鸡尿沉渣中针状及球状晶体含量相当。与对照组相比,高钙组尿沉渣中钙、磷含量升高,而钾、钠和镁含量均下降;高蛋白组和高钙高蛋白组尿沉渣中磷含量升高,而钙、钾和钠含量均下降。

One hundred and twenty 35 days of age, healthy growing layers were randomly divided into four groups. The growers in different groups were fed with normal calcium and crude protein diet, high calcium and normal protein diet, normal calcium and high protein diet and high calcium and high protein diet respectively. At 50 d of age, pullets in four groups were colostomized to collect the urine, studying urine and urinary deposit. The result showed typical visceral gout in growers was successfully induced by HCHP diet. Growers raised on HCHP and HC diet had significantly higher urine pH and larger volume of urine and lower the relative contents of urinary calcium, sodium,＇potassium and magnesium and total quantity of 24 h urinary excretion of sodium than that of the control and HP. For HC growers and HCHP growers were significantly lower when compared to the control and HP growers. The growers raised on HC and HCHP diet had significantly higher total quantity of 24 h urinary excretion of uric acid, calcium, magnesium, inorganic phosphorus, potassium in urine than that of the control. The relative contents of inorganic phosphorus and total quantity of 24 h urinary excretion of inorganic phosphorus and uric acid for HP growers were significantly higher when compared with the control growers. The urinary deposit of control and HP group showed large amount of scattered spheres. The urinary deposit of high calcium group showed large amount of crystals of needlelike. The urinary deposit of high calcium and high protein groups showed large amount of crystals of needlelike and scattered spheres structure. calcium, phosphorus increased and potassium, sodium, magnesium decreased in the urinary deposit in the HC group, meanwhile, phosphorus increased and calcium, potassium, sodium decreased in the HP and HCHP group as compared with the control group.