均匀设计优选南板蓝根多糖的提取工艺

Study on the Optimal Extraction Process of Polysaccharide from Baphicacanthus cuaia (Nees) Bremek with the Uniform Design

目的:优选南板蓝根中多糖的提取工艺。方法:用均匀设计方法设计水提取工艺,以提取物中多糖含量为测定指标,考察提取温度,提取时间,料液比三个因素对有效成分多糖提取的影响。结果:最佳工艺为提取温度90℃,提取时间2h,料液比1∶50(g/ml),南板蓝根多糖的提取率为3·26%。在此条件下,北板蓝根多糖的提取率为12·76%。

Objective： To study the optimal extraction process of polysaccharide from Baphicacanthus cuaia （Nees） Bremek. Methods： The optimal extraction process was selected with uniform design and statistical analysis system （SAS）. The content of polysaccharide in the extract was determined to investigate the influence of temperature of extraction, the time of extraction and the solid-liquid ratio on it. Results： The optimal extraction process was extraction temperature 90℃ with 2 hours and with 1：50 （g/ml） of Baphicacanthus cuaia （Nees） Bremek weight to distilled water. The optimum extraction rates of Polysaccharide from Baphicacanthus cuaia （Nees） Bremek and Isatis indigotica Fort were 3.26% and 12. 76%.