荧光定量PCR法检测鼻咽癌血浆游离EBV DNA的临床意义

Clinical significance of Plasma Epstein-Bar virus DNA concentration in nasopharyngeal carcinoma with fluorescent quantitative PCR

目的通过检测血浆中游离EBVDNA水平,探讨其在鼻咽癌诊断、鉴别诊断、临床分期中的临床意义。方法采用荧光定量PCR方法定量检测111例初治鼻咽癌患者和107例放疗后持续缓解患者血浆游离EBVDNA水平。结果初治组及临床缓解组血浆中游离EBVDNA检出率分别为74.8%(83/111)和4.6%(5/107),初治组检出率显著高于临床缓解组,在统计学上有显著性差异;初治组中晚期(Ⅲ+Ⅳ期)血浆游离EBVDNA拷贝数显著高于早期(Ⅰ+Ⅱ期);N晚期(N2+N3)拷贝数显著高于早期(N0+N1);T晚期(T3+T4)拷贝数显著高于早期(T1+T2);统计学上均有显著性差异。结论荧光定量PCR方法检测血浆中游离EBVDNA是一种敏感可靠的方法,血浆游离EBVDNA定量检测对鼻咽癌诊断、鉴别诊断、分期、治疗后监测有重要的临床意义,有望成为鼻咽癌肿瘤学标记物。

Objective To study the clinical significance of plasma EBV DNA concentration in diagnosis.differential diagnosis,clinical staging in nasopharyngeal carcinoma.Methods Using fluorescent quantitative PCR.the level of plasma EBV DNA were detected in 111 patients with primary NPC and 107 patients with, cliaical remission after radiotberapy.Results Plasma EBV DNA was detectable in 74.8%（83/111 ）of primary NPC and 4.6%（5/107）of clinical remission parle,Its. there was a significant difference statistically in the detectable proportion betwee,1 the two groups. In primary NPC .patients in advanced TNM stage had signifieantly higher plasma EBV DNA level than those of in early TNM stage.Conclusion Fluorescent quantitative PCR in detecting EBV DNA is a sensitive.reliable method,the quantification of plasma EBV DNA is of sifnificance in diagnosis,differential diagnosis.clinical staging and monitoring after treatment.It is possible that plasma EBV DNA should become a serologic marker of NPC.