大黄鱼消化道内分泌细胞免疫组织化学的定位

Immunohistochemical localization of endocrine cells in the digestive tract of Pseudosciaena crocea

应用过氧化物酶标记的链霉亲和素免疫组织化学技术(简称S-P法),用抗5-羟色胺(5-HT)、生长抑素(SST)、胃泌素(Gas)、胰高血糖素(Glu)等4种抗哺乳动物血清对大黄鱼(Pseudosciaena crocea)消化道内分泌细胞进行免疫组织化学定位研究,结果表明：5-HT免疫阳性细胞在消化道各段均有分布,胃各部分布较多,食管和肠管分布较少；SST在胃贲门部和胃体部数量较多,胃幽门部较少,食管、前肠和中肠仅有少量分布,后肠的分布数量多。Gas分布于胃幽门部和前肠、中肠、后肠,从胃幽门部到后肠呈递减分布趋势；食管、胃贲门部和胃体部均未检出。Glu免疫阳性细胞在消化道各段均未发现。根据形态学特征,大黄鱼消化道内分泌细胞与其他鱼类一样,分为两种类型,即开放型和闭合型细胞。

The immunohistochemical localization of endocrine cells in the digestive tract of Pseudosciaena crocea were studied with four mammal serum i.e. anti-5-HT, anti-SST, anti-Gas and anti-Glu by the immunohistochemical technique of the streptavidin-peroxidase marked with HRP. The results showed that the immunologically positive endocrine cells of 5-HT were distributed throughout the digestive tract, and the distributive density of 5-HT cell was higher throughout stomach, less in oesophagus and intestine. The immunologically positive endocrine cells of SST were located mostly in stomachus cardiacus, and stomachus fundus, but less in stomachus pyloricu, and only a few in the oesophagus, foregut and midgut, and it increased in hidgut. The immunologically positive endocrine cells of Gas were found in stomachus pyloricu, foregut and midgut, hidgut. It indicated that the cell density reduced from stomachus pyloricu to hidgut, but it was not found in oesophagus, stomachus cardiacus, and stomachus fundus. The immunologically positive endocrine cells of Glu were not found throughout the digestive tract. According to the morphologic character, the endocrine cells in the digestive tract of Pseudosciaena crocea were the same as other kinds of fish. It could be divided into two cell types, the opening and the closing types.