摘要
对长链烷烃降解菌的降解能力和摄取模式进行了研究。评价14株烃降解菌利用中长链烃生长的能力,发现只有少数烃降解菌能够获得良好生长,其中Mycobacterium fortuitum514,Pseudomonas aeruginosa1785和Pseudomonas marginata766等3株菌能够高效降解C20到C33的长链烷烃。辛烷不能支持这些长链烷烃降解菌的生长,说明其烃氧化酶与Pseudomonas oleovorans的OCT质粒编码的单氧酶不同。此外,M.fortuitum不产胞外表面活性剂,而P.aeruginosa和P.marginata则是表面活性剂产生菌,然而三者在以烃为碳源生长时均显示出很高的细胞表面疏水性。根据生长现象分析3株菌采用了不同的烷烃摄取模式。
The degradation ability and uptake modes of Long-chain alkane-degrading bacteria were investigated. Among 14 bacterial species belonging to hydrocarbon-degrading bacteria, only a few strains were capable of utilizing long chain alkanes as the sole carbon source. Mycobacterium fortuitum 514, Pseudomonas aeruginosa 1785 and Pseudomonas marginata 766 showed the exceptional capability of degrading long-chain aliphatic alkanes ranging from C40 to C,33, Octane can not supported the growth of these strains, which indicate those strains possess alkane hydroxylases differing from the monooxygenase encoded on the well-known OCT plasmid of Pseudomonas oleovorans Gpol. M. fortuitum 514 was deficient to produce extracellular surfactant, while P. aeruginosa 1785 and P. marginata 766 were surfactant producer. High cell surface hydrophobicty was observed for each strain grown on alkanes. These studies supported the conclusion that different uptake modes were adopted by alkane degrading bacteria.
出处
《微生物学杂志》
CAS
CSCD
2005年第6期14-18,共5页
Journal of Microbiology
基金
国家自然科学基金(50374038)
高等学校博士学科点专项科研基金(20030251002)
教育部重点项目(03071)和上海市教委资助项目
关键词
长链烷烃
烃降解菌
摄取模式
Long-chain alkane
hydrocarbon-degrading bacteria
uptake mode